Journal article
Integrated immunochromatographic strip with glucometer readout for rapid quantification of phosphorylated proteins
Analytica chimica acta, Vol.964, pp.1-6
04/29/2017
Handle:
https://hdl.handle.net/2376/109099
PMID: 28351626
Abstract
A new technology to quantify phospho-p5315 by converting its content to the amount of glucose which is detectable by a glucometer was developed. An immunochromatographic test strip (ITS) was used as a disposable platform, where primary antibody (Ab1)-modified Fe3O4 magnetic nanoparticles (Fe3O4-Ab1) were settled on the test zone to capture both the target phospho-p5315 and the detection antibody (Ab2)-glucose encapsulating liposome (GEL) conjugate. The measurement was based on the release and subsequent detection of glucose from Ab2-GEL using a glucose meter (GM). The amount of glucose is proportional to the phospho-p5315 concentration from 0.1 to 50 ng mL−1, the limit of detection is 50 pg mL−1 (3S/N). The high sensitivity was a result of the huge number of glucose encapsulated in the liposome. Taking the advantage of low cost, widespread availability and portability of the test trip, together with the personal GM, the presented approach can be easily used to detect other disease biomarkers in medical diagnostics and environmental monitoring.
A new technology to quantify phospho-p5315 by converting its content to the amount of glucose which is detectable by a glucometer was developed. An immunochromatographic test strip (ITS) was used as a disposable platform. The measurement was based on the release and subsequent detection of glucose from Ab2-GEL using a glucose meter (GM). Taking the advantage of low cost, widespread availability and portability of the test trip, together with the personal GM, the presented approach can be easily used to detect other disease biomarkers in medical diagnostics and environmental monitoring. [Display omitted]
•A new technology to quantify phosphorylated proteins based on integration of immunochromatographic strip with glucometer.•The novelty lies in converting the quantification of target biomarker to the detection of glucose amount.•The high sensitivity results from glucose encapsulating liposomes for signal amplification.•The platform is easily developed into a universal method applicable for the quantification of broad biomarkers.
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Details
- Title
- Integrated immunochromatographic strip with glucometer readout for rapid quantification of phosphorylated proteins
- Creators
- Yuting Zhao - Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaXiao Chen - Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaSophie Lin - School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USADan Du - Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR ChinaYuehe Lin - School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Analytica chimica acta, Vol.964, pp.1-6
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99900547393501842
- Language
- English
- Resource Type
- Journal article