Journal article
Interaction between myosin heavy chain and troponin isoforms modulate cardiac myofiber contractile dynamics
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.293(4), pp.R1595-1607
10/2007
Handle:
https://hdl.handle.net/2376/113805
PMID: 17626127
Abstract
Coordinated expression of species-specific myosin heavy chain (MHC) and troponin (Tn) isoforms may bring about a dynamic complementarity to match muscle contraction speed with species-specific heart rates. Contractile system function and dynamic force-length measurements were made in muscle fibers from mouse and rat hearts and in muscle fibers after reconstitution with either recombinant homologous Tn or orthologous Tn. The rate constants of length-mediated cross-bridge (XB) recruitment (b) and tension redevelopment (k(tr)) of mouse fibers were significantly faster than those of rat fibers. Both the tension cost (ATPase/tension) and rate constant of length-mediated XB distortion (c) were higher in the mouse than in the rat. Thus the mouse fiber was faster in all dynamic and functional aspects than the rat fiber. Mouse Tn significantly increased b and k(tr) in rat fibers; conversely, rat Tn significantly decreased b and k(tr) in mouse fibers. Thus the length-mediated recruitment of force-bearing XB occurs much more rapidly in the presence of mouse Tn than in the presence of rat Tn, demonstrating that the speed of XB recruitment is regulated by Tn. There was a significant interaction between Tn and MHC such that changes in either Tn or MHC affected the speed of XB recruitment. Our data demonstrate that the dynamics of myocardial contraction are different in the mouse and rat hearts because of sequence heterogeneity in MHC and Tn. At the myofilament level, coordinated expression of complementary regulatory contractile proteins produces a functional dynamic phenotype that allows the cardiovascular systems to function effectively at different heart rates.
Metrics
6 Record Views
Details
- Title
- Interaction between myosin heavy chain and troponin isoforms modulate cardiac myofiber contractile dynamics
- Creators
- Murali Chandra - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University, 205 Wegner Hall, Pullman, Washington 99164, USA. murali@vetmed.wsu.eduMatthew L TschirgiSteven J FordBryan K SlinkerKenneth B Campbell
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.293(4), pp.R1595-1607
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- United States
- Grant note
- R01 HL-075643-02 / NHLBI NIH HHS
- Identifiers
- 99900547531701842
- Language
- English
- Resource Type
- Journal article