Journal article
Intercellular adhesion molecule-1 expression induced by interleukin (IL)-1β or an IL-1β fragment is blocked by an IL-1 receptor antagonist and a soluble IL-1 receptor
Journal of neuroimmunology, Vol.44(2), pp.163-170
1993
Handle:
https://hdl.handle.net/2376/104056
PMID: 8099361
Abstract
The effects of a recombinant human interleukin-1 (IL-1) receptor antagonist (IL-1ra) and a recombinant human soluble IL-1 receptor (sIL-1R) on cytokine-induced intercellular adhesion molecule-1 (ICAM-1) expression in a human glioblastoma cell line and a neuroblastoma cell line were determined. Cells were incubated with IL-1β, tumor necrosis factor (TNF)α and interferon (IFN)γ. Cells were also tested under identical conditions with an IL-1β synthetic peptide fragment (IL-1β
208–240) previously shown to possess biological activity. IL-1β, TNFα and IFNγ potentiated ICAM-1 expression in both cell lines in a dose-related manner. The IL-1β
208–240 fragments, corresponding to the rabbit, rat and human sequences, enhanced ICAM-1 expression in glioblastoma cells at high doses. ICAM-1 expression induced by IL-1β, rabbit IL-1β
208–240 and human IL-1β
208–240 was blocked by the IL-1ra, while TNFα- and IFNγ-induced ICAM-1 expression were not. ICAM-1 expression induced by IL-1β and human IL-1β
208–240 was also blocked by the sIL-1R. Our findings suggest that IL1β
208–240 acts as an IL-1β agonist in enhancing ICAM-1 expression in vitro and that this effect is receptor-mediated.
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Details
- Title
- Intercellular adhesion molecule-1 expression induced by interleukin (IL)-1β or an IL-1β fragment is blocked by an IL-1 receptor antagonist and a soluble IL-1 receptor
- Creators
- Lielie Hong - Department of Physiology and Biophysics University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USALuca Imeri - Department of Physiology and Biophysics University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USAMark R Opp - Department of Physiology and Biophysics University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USAArnold E Postlethwaite - Department of Medicine University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USAJerome M Seyer - Department of Biochemistry, University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USAJames M Krueger - Department of Physiology and Biophysics University of Tennessee and Veterans Administration Medical Center, Memphis, TN, USA
- Publication Details
- Journal of neuroimmunology, Vol.44(2), pp.163-170
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546821701842
- Language
- English
- Resource Type
- Journal article