Journal article
Interferon- γ and interleukin-12 mediate protection to acute Neospora caninum infection in BALB/c mice
International journal for parasitology, Vol.29(10), pp.1635-1646
1999
Handle:
https://hdl.handle.net/2376/114290
PMID: 10608450
Abstract
The type of immune response required to protect mice against clinical disease during acute
Neospora caninum challenge was investigated in BALB/c mice. Groups of female BALB/c mice were infected i.p. with
N. caninum tachyzoites concomitant with either: (1) antibody to interferon-
γ; (2) recombinant murine interleukin-12; or (3) recombinant murine interleukin-12 plus antibody to interferon-
γ. Mice treated with anti-interferon-
γ alone had increased morbidity/mortality, decreased body weight, increased foci of liver necrosis and increased numbers of
N. caninum tachyzoites in the lung by 7
days p.i. compared with controls. Increased disease and parasite load in the anti-interferon-
γ-treated mice was associated with antigen-specific antibody IgG1>IgG2a and a three-fold decreased ratio of antigen-specific interferon-
γ
:
interleukin-4. Mice treated with recombinant murine interleukin-12 had decreased encephalitis and brain parasite load at 3
weeks p.i. compared with control mice treated with PBS. In recombinant murine interleukin-12-treated mice, decreased brain lesions and parasite load were associated with antigen-specific antibody IgG2a>IgG1 and a three-fold increased ratio of antigen-specific interferon-
γ
:
interleukin-4 from splenocytes; the interleukin-12 effect was dependent upon interferon-
γ, as indicated by concomitant in vivo interferon-
γ neutralisation. By 6
weeks p.i. with
N. caninum, there were no differences in brain lesions and parasite load between interleukin-12- and PBS-treated groups, indicating that the effects of interleukin-12 on driving a protective type 1 response were transient. These data indicate a role for interferon-
γ, interleukin-12 and type 1 immune responses in control of acute neosporosis in mice.
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Details
- Title
- Interferon- γ and interleukin-12 mediate protection to acute Neospora caninum infection in BALB/c mice
- Creators
- Timothy V BaszlerMaureen T LongTerry F McElwainBruce A Mathison
- Publication Details
- International journal for parasitology, Vol.29(10), pp.1635-1646
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547981901842
- Language
- English
- Resource Type
- Journal article