Journal article
Interleukin-12 as an Adjuvant Promotes Immunoglobulin G and Type 1 Cytokine Recall Responses to Major Surface Protein 2 of the Ehrlichial Pathogen Anaplasma marginale
Infection and immunity, Vol.68(1), pp.270-280
01/2000
Handle:
https://hdl.handle.net/2376/114052
PMCID: PMC97131
PMID: 10603398
Abstract
Anaplasma marginale is a tick-transmitted pathogen of cattle closely related to the human ehrlichiae, Ehrlichia chaffeensis and the agent of human granulocytic ehrlichiosis (HGE). These pathogens have in common a structurally conserved outer membrane protein (OMP) designated the major surface protein 2 (MSP-2) in A. marginale and HGE and OMP-1 in E. chaffeensis . Protective immunity against ehrlichial pathogens is believed to require induction of gamma interferon (IFN-γ) and opsonizing immunoglobulin (Ig) subclasses directed against OMP epitopes that, in concert, activate macrophages for phagocytosis and killing. Because interleukin-12 (IL-12) acts as an adjuvant for protein immunization to induce IFN-γ and protective immunity against intracellular pathogens, we hypothesized that as an adjuvant with MSP-2, IL-12 would augment type 1 recall responses to A. marginale . IL-12 was coadsorbed with MSP-2 to alum and shown to significantly enhance IFN-γ production by lymph node cells (LNC) and LNC-derived CD4 + T-cell lines from immunized calves following recall stimulation with A. marginale . LNC proliferation and IL-2 production were also enhanced in IL-12-treated calves. Elevated recall proliferative responses by peripheral blood mononuclear cells were still evident 9 months after immunization. Serum IgG levels were consistently increased in IL-12 immunized calves, predominantly due to higher IgG1 responses. The results support the use of IL-12 coadsorbed with OMP of ehrlichial pathogens in alum to amplify both antibody and type-1 cytokine responses important for protective immunity.
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Details
- Title
- Interleukin-12 as an Adjuvant Promotes Immunoglobulin G and Type 1 Cytokine Recall Responses to Major Surface Protein 2 of the Ehrlichial Pathogen Anaplasma marginale
- Creators
- Wenbin Tuo - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Guy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Travis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Daming Zhu - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Wendy C Brown - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Infection and immunity, Vol.68(1), pp.270-280
- Academic Unit
- Paul G. Allen School for Global Animal Health; Department of Veterinary Microbiology and Pathology
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547699001842
- Language
- English
- Resource Type
- Journal article