Journal article
Interleukin-15 activated bovine natural killer cells express CD69 and produce interferon-γ
Veterinary immunology and immunopathology, Vol.150(1-2), pp.79-89
11/15/2012
Handle:
https://hdl.handle.net/2376/113439
PMID: 23021353
Abstract
Interleukin (IL)-15 is an essential cytokine in natural killer (NK) cell development and survival. In humans, IL-15 shows overlapping properties with IL-2 due to partly shared receptors and signal transduction and both cytokines synergize equally well with IL-12 in the induction of interferon (IFN)-γ production from NK cells. Bovine NK cells however, have been reported to produce less IFN-γ after in vitro IL-12 stimulation when exposed to human IL-15 in comparison to bovine IL-2. We therefore wanted to determine if homologous IL-15 is needed for adequate stimulation of bovine NK cells. Biologically active recombinant bovine IL-15 (rbIL-15) produced in mammalian cells by the use of a modified expression vector stimulated NK cells to a dose-dependent IFN-γ production in the presence of IL-12. In contrast to earlier findings, we also detected potent IFN-γ production from bovine NK cells stimulated by human IL-15 and IL-12.
Finally, we describe a monoclonal antibody recognizing bovine CD69 and show the expression of this early activation marker on bovine NK cells ex vivo and following rbIL-15 stimulation.
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Details
- Title
- Interleukin-15 activated bovine natural killer cells express CD69 and produce interferon-γ
- Creators
- Hege Lund - Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, NorwayPreben Boysen - Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, NorwayGregg A Dean - Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1619, USAWilliam C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAKun Taek Park - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAAnne K Storset - Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, Norway
- Publication Details
- Veterinary immunology and immunopathology, Vol.150(1-2), pp.79-89
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547653501842
- Language
- English
- Resource Type
- Journal article