Journal article
Interleukin-1beta induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP
Brain research. Molecular brain research., Vol.137(1-2), pp.213-222
06/13/2005
Handle:
https://hdl.handle.net/2376/105649
PMID: 15950780
Abstract
Interleukin-1 beta (IL-1) and CREB have many CNS actions including sleep regulation and hippocampal-dependent learning. CREB acts in part via CREB-binding protein (CBP). We thus determined whether IL-1 could induce CBP gene expression. Initially, cultured hippocampal cells were treated with IL-1 and differential display reverse transcription was used to identify up- and down-regulated genes. We then sequenced rat CBP. Of the IL-1-upregulated genes, CBP and adenine nucleotide translocator-1 (ANT-1) were investigated in vivo. In these experiments, IL-1 was given to rats intraventricularly and sacrificed 2 h later; both CBP and ANT-1 transcripts were upregulated in the cerebral cortex and hypothalamus. We conclude that rat CBP shares many of the functional domains as human and murine CBP and that IL-1 upregulates genes previously associated with learning and sleep.
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Details
- Title
- Interleukin-1beta induces CREB-binding protein (CBP) mRNA in brain and the sequencing of rat CBP
- Creators
- Chad Tang - Department of VCAPP, Program in Neuroscience, Washington State University, PO Box 646520, Pullman, WA 99164-6520, USAMee Ja SulaStewart BohnetAbdur RehmanPing TaishiJames M Krueger
- Publication Details
- Brain research. Molecular brain research., Vol.137(1-2), pp.213-222
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Netherlands
- Grant note
- NS 31453 / NINDS NIH HHS NS 25378 / NINDS NIH HHS
- Identifiers
- 99900547056001842
- Language
- English
- Resource Type
- Journal article