Journal article
Intermolecular relationships of major surface proteins of Anaplasma marginale
Infection and immunity, Vol.62(7), pp.2940-2946
07/1994
Handle:
https://hdl.handle.net/2376/100637
PMCID: PMC302901
PMID: 8005681
Abstract
Immunization with Anaplasma marginale membranes containing major surface proteins (MSPs) induces protective immunity against clinical disease (N. Tebele, T. C. McGuire, and G. H. Palmer, Infect. Immun. 59:3199-3204, 1991). For use in design of a recombinant antigen subunit vaccine for A. marginale, intermolecular relationships of known A. marginale MSPs were analyzed. Under nonreducing conditions, MSP-2 and MSP-5 occur as multimers. A large (> 300-kDa-molecular-mass), nonreduced protein complex contained MSP-1a linked by disulfide bonds to MSP-1b and by noncovalent bonds to MSP-5. MSP-2 was also noncovalently bound to this complex. The nearest neighbor membrane proteins were identified by cross-linking reactions followed by immunoblotting with anti-MSP antibodies. A cross-linked aggregate retained in the stacking gel contained MSP-1a, MSP-1b, MSP-2, MSP-3, MSP-4, and MSP-5. Collectively, the data indicate that MSP-2 and MSP-5 occur as monomers and disulfide-bonded multimers. The MSP-1 complex occurs as both disulfide-bonded and noncovalently associated MSP-1 and MSP-1b, and MSP-2 and MSP-5 are noncovalently associated with MSP-1. Also, MSP-1, MSP-2, MSP-3, and MSP-4 are nearest neighbors, and MSP-5 is noncovalently associated with this cross-linked complex.
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Details
- Title
- Intermolecular relationships of major surface proteins of Anaplasma marginale
- Creators
- M C Vidotto - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040T C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040T F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040G H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040D P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040
- Publication Details
- Infection and immunity, Vol.62(7), pp.2940-2946
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546696801842
- Language
- English
- Resource Type
- Journal article