Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme

Tong Ihn Lee; John J Wyrick; Sang Seok Koh; Ezra G Jennings; Ellen L Gadbois; Richard A Young

doi:10.1128/MCB.18.8.4455

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Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme

Journal article

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Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme

Tong Ihn Lee, John J Wyrick, Sang Seok Koh, Ezra G Jennings, Ellen L Gadbois and Richard A Young

Molecular and cellular biology, Vol.18(8), pp.4455-4462

08/1998

DOI: https://doi.org/10.1128/MCB.18.8.4455

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https://hdl.handle.net/2376/110634

PMCID: PMC109031

PMID: 9671455

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Abstract

Transcriptional Regulation

Activation of protein-encoding genes involves recruitment of an RNA polymerase II holoenzyme to promoters. Since the Srb4 subunit of the holoenzyme is essential for expression of most class II genes and is a target of at least one transcriptional activator, we reasoned that suppressors of a temperature-sensitive mutation in Srb4 would identify other factors generally involved in regulation of gene expression. We report here that MED6 and SRB6 , both of which encode essential components of the holoenzyme, are among the dominant suppressors and that the products of these genes interact physically with Srb4. The recessive suppressors include NCB1 (BUR6) , NCB2 , NOT1 , NOT3 , NOT5 , and CAF1 , which encode subunits of NC2 and the Not complex. NC2 and Not proteins are general negative regulators which interact with TATA box binding protein (TBP). Taken together, these results suggest that transcription initiation involves a dynamic balance between activation mediated by specific components of the holoenzyme and repression by multiple TBP-associated regulators.

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Title: Interplay of Positive and Negative Regulators in Transcription Initiation by RNA Polymerase II Holoenzyme
Creators: Tong Ihn Lee - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
John J Wyrick - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Sang Seok Koh - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Ezra G Jennings - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Ellen L Gadbois - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Richard A Young - Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Publication Details: Molecular and cellular biology, Vol.18(8), pp.4455-4462
Academic Unit: Molecular Biosciences, School of
Publisher: American Society for Microbiology
Identifiers: 99900547107601842
Language: English
Resource Type: Journal article