Journal article
Intracellular free calcium in response to oxytocin in pig endometrial cells
Molecular and cellular endocrinology, Vol.155(1), pp.77-83
1999
Handle:
https://hdl.handle.net/2376/108674
PMID: 10580841
Abstract
Intracellular free calcium concentration ([Ca
2+]
i) in response to oxytocin (OT) was studied in stromal, glandular epithelial and luminal epithelial cells obtained from the endometrium of gilts 16 days post-estrus. The amplitude of increased [Ca
2+]
i in response to 100 nM OT was greatest in stromal cells, intermediate in glandular epithelial cells and not evident in luminal epithelial cells. During continuous OT administration, stromal cells responded initially with a synchronous spike of [Ca
2+]
i that did not require extracellular Ca
2+ and then displayed spontaneous asynchronous [Ca
2+]
i spikes that required extracellular Ca
2+. Each cell possessed its own characteristic response. Increasing concentrations of OT induced an increasing percentage of stromal cells responding, with some cells having nearly equal [Ca
2+]
i responses at all concentrations and others having graded [Ca
2+]
i responses as the concentration of OT increased. These results are consistent with the proposed mechanism of OT action in pig endometrium involving activation of phosphoinositide-Ca
2+ signaling pathway.
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Details
- Title
- Intracellular free calcium in response to oxytocin in pig endometrial cells
- Creators
- Gheorghe T Braileanu - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6353, USASteven M Simasko - Center for Reproductive Biology, Washington State University, Pullman, WA 99164-4231, USAMehmet Uzumcu - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6353, USAMark A Mirando - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6353, USA
- Publication Details
- Molecular and cellular endocrinology, Vol.155(1), pp.77-83
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900547513101842
- Language
- English
- Resource Type
- Journal article