Journal article
Intracellular localization of Brucella abortus and Francisella tularensis in primary murine macrophages
Methods in molecular biology (Clifton, N.J.), Vol.431, pp.133-145
2008
Handle:
https://hdl.handle.net/2376/106706
PMID: 18287753
Abstract
Intracellular bacterial pathogens have evolved sophisticated strategies to survive and proliferate within cells of their hosts. Studying their intracellular life cycle is key to understanding virulence and requires methodologies that can identify the compartments in which they localize and characterize the replicative niche they generate. Here, we describe immunofluorescence-based microscopy techniques applied to the intracellular pathogens Brucella abortus and Francisella tularensis during their respective intracellular cycles inside murine bone marrow-derived macrophages. Standard immunofluorescence techniques are used to define the intracellular localization of the pathogens based on their co-localization with specifically labeled macrophage organelles. In addition, we describe an assay to assess the integrity of Francisella-containing phagosomes and bacterial release into the macrophage cytoplasm, which is a hallmark of Francisella intracellular pathogenesis.
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Details
- Title
- Intracellular localization of Brucella abortus and Francisella tularensis in primary murine macrophages
- Creators
- Jean Celli - Tularemia Pathogenesis Section, Laboratory of Parasites, Rocky Mountain Laboratoties, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.431, pp.133-145
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- Intramural NIH HHS
- Identifiers
- 99900546816001842
- Language
- English
- Resource Type
- Journal article