Journal article
Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body
The Journal of cell biology, Vol.164(2), pp.255-266
01/19/2004
Handle:
https://hdl.handle.net/2376/108280
PMID: 14718520
Abstract
Intraflagellar transport (IFT) is the bidirectional movement of multisubunit protein particles along axonemal microtubules and is required for assembly and maintenance of eukaryotic flagella and cilia. One posited role of IFT is to transport flagellar precursors to the flagellar tip for assembly. Here, we examine radial spokes, axonemal subunits consisting of 22 polypeptides, as potential cargo for IFT. Radial spokes were found to be partially assembled in the cell body, before being transported to the flagellar tip by anterograde IFT. Fully assembled radial spokes, detached from axonemal microtubules during flagellar breakdown or turnover, are removed from flagella by retrograde IFT. Interactions between IFT particles, motors, radial spokes, and other axonemal proteins were verified by coimmunoprecipitation of these proteins from the soluble fraction of
Chlamydomonas
flagella. These studies indicate that one of the main roles of IFT in flagellar assembly and maintenance is to transport axonemal proteins in and out of the flagellum.
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Details
- Title
- Intraflagellar transport (IFT) cargo
- Creators
- Hongmin Qin - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520Dennis R Diener - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520Stefan Geimer - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520Douglas G Cole - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520Joel L Rosenbaum - Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520
- Publication Details
- The Journal of cell biology, Vol.164(2), pp.255-266
- Academic Unit
- Center for Reproductive Biology
- Publisher
- The Rockefeller University Press
- Identifiers
- 99900547649201842
- Language
- English
- Resource Type
- Journal article