Journal article
Investigation of enzyme formulation on pretreated switchgrass
Bioresource technology, Vol.102(24), pp.11072-11079
2011
Handle:
https://hdl.handle.net/2376/106471
PMID: 21478012
Abstract
This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime
+
ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO
2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0–2%) for the pretreatments with low xylan content (dilute-acid, SO
2). Xylanase addition also improved overall yields with lime
+
ball-milling and SO
2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime
+
ball-milling obtained an enzymatic yield of 92.3
kg of sugar digested/kg of protein loaded.
Metrics
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Details
- Title
- Investigation of enzyme formulation on pretreated switchgrass
- Creators
- Matthew Falls - Texas A&M University, College Station, TX 77843, United StatesJian Shi - University of California, Riverside, CA 95207, United StatesMirvat A Ebrik - University of California, Riverside, CA 95207, United StatesTim Redmond - University of California, Riverside, CA 95207, United StatesBin Yang - University of California, Riverside, CA 95207, United StatesCharles E Wyman - University of California, Riverside, CA 95207, United StatesRebecca Garlock - Michigan State University, East Lansing, MI 48824, United StatesVenkatesh Balan - Michigan State University, East Lansing, MI 48824, United StatesBruce E Dale - Michigan State University, East Lansing, MI 48824, United StatesV. Ramesh Pallapolu - Auburn University, Auburn, AL 36849, United StatesY.Y Lee - Auburn University, Auburn, AL 36849, United StatesYoungmi Kim - Purdue University, West Lafayette, IN 47907, United StatesNathan S Mosier - Purdue University, West Lafayette, IN 47907, United StatesMichael R Ladisch - Purdue University, West Lafayette, IN 47907, United StatesBonnie Hames - Ceres, Inc., Thousand Oaks, CA 91320, United StatesSteve Thomas - Ceres, Inc., Thousand Oaks, CA 91320, United StatesBryon S Donohoe - National Renewable Energy Laboratory, Golden, CO 80401, United StatesTodd B Vinzant - National Renewable Energy Laboratory, Golden, CO 80401, United StatesRichard T Elander - National Renewable Energy Laboratory, Golden, CO 80401, United StatesRyan E Warner - Genencor, A Danisco Division, Palo Alto, CA 94304, United StatesRocio Sierra-Ramirez - Universidad de los Andes, Grupo de Conversion de Energia, Bogotá, ColombiaMark T Holtzapple - Texas A&M University, College Station, TX 77843, United States
- Publication Details
- Bioresource technology, Vol.102(24), pp.11072-11079
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546737301842
- Language
- English
- Resource Type
- Journal article