Journal article
Investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay
Journal of clinical microbiology, Vol.33(8), pp.2048-2053
08/1995
Handle:
https://hdl.handle.net/2376/104925
PMCID: PMC228333
PMID: 7559946
Abstract
Development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (SA-MCF) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. A recently developed competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA) for the detection of antibody to malignant catarrhal fever (MCF) virus (MCFV) in ruminants based on a monoclonal antibody to a widely conserved epitope of MCFV (H. Li, D. T. Shen, D. P. Knowles, J. R. Gorham, and T. B. Crawford, J. Clin. Microbiol. 32:1674-1679, 1994) and a PCR assay based on previously reported primers (S. I. F. Baxter, I. Pow, A. Bridgen, and H. W. Reid, Arch. Virol. 132:145-159, 1993) were used to detect anti-MCFV antibody and SA-MCFV DNA in sheep and other ruminants. The PCR amplified a specific 238-bp SA-MCFV genomic DNA fragment from peripheral blood lymphocytes of adult sheep and other ruminants with clinical MCF. Of 144 samples from randomly selected healthy adult sheep, 143 (99%) were positive by PCR and 136 (94%) were positive by CI-ELISA. The agreement between the two assays exceeded 95%. Of nine samples collected from cattle and deer with clinical MCF of apparent sheep origin, seven were CI-ELISA positive and all 9 were PCR positive. Among 59 serum samples from presuckling lambs, none contained antibody detectable by CI-ELISA. After suckling, maternal anti-MCFV antibody was detectable for about 10 +/- 3 weeks.
Metrics
5 Record Views
Details
- Title
- Investigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay
- Creators
- H Li - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USAD T Shen - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USAD O'Toole - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USAD P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USAJ R Gorham - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USAT B Crawford - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA
- Publication Details
- Journal of clinical microbiology, Vol.33(8), pp.2048-2053
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900546778201842
- Language
- English
- Resource Type
- Journal article