Journal article
Investigation of wheat straw biodegradation by Phanerochaete chrysosporium
Biomass & bioenergy, Vol.35(3), pp.1030-1040
03/2011
Handle:
https://hdl.handle.net/2376/117846
Abstract
Production of renewable fuels and chemicals from biomass requires an efficient pretreatment technology, which further depends on better understanding of biodegradation process of such lignocellulosic biomass. The biodegradation of wheat straw by Phanerochaete chrysosporium was investigated in this study. The fungal secretomes and compositional, functional groups and structural changes of the fungal spent wheat straw lignin were determined. The result showed ∼ 30% loss of total lignin within three weeks of biopretreatment by P. chrysosporium. Detailed structural analysis through two dimentional heteronuclear multiple quantum coherence nuclear magnatic resonance (2D HMQC NMR) of the pretreated lignin (acetylated) revealed low abundance of substructures D (dibenzodioxacin) and E (cinnamyl alcohol). Further, analysis of lignin by Fourier Transmission Infrared (FTIR) and Pyrolysis Gas Chromatography/Mass Spectrometry (Py-GC/MS) demonstrated the significant decrease of guaiacyl (G) units. The results support the previous findings in the biodegradation of wheat straw analyzed by 13C cross polarization magic angle spinning (CPMAS). Revealing the characteristic behavior of P. chrysosporium mediated biomass degradation, the information presented in this paper offers new insights for understanding the biological lignin degradation of wheat straw by P. chrysosporium.
Metrics
13 Record Views
Details
- Title
- Investigation of wheat straw biodegradation by Phanerochaete chrysosporium
- Creators
- Deepak Singh - Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman, WA 99163, USAJijiao Zeng - Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman, WA 99163, USADhrubojyoti D Laskar - Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman, WA 99163, USALee Deobald - Environmental Biotechnology Institute, University of Idaho, USAWilliam C Hiscox - NMR center, Washington State University, USAShulin Chen - Department of Biological Systems Engineering, The Bioprocessing and Bioproduct Engineering Laboratory (BBEL), Washington State University, L.J. Smith 213, Pullman, WA 99163, USA
- Publication Details
- Biomass & bioenergy, Vol.35(3), pp.1030-1040
- Academic Unit
- Biological Systems Engineering, Department of; NMR Center
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548586301842
- Language
- English
- Resource Type
- Journal article