Journal article
Involvement of ROP6 and clathrin in nodulation factor signaling
Plant signaling & behavior, Vol.10(7), pp.e1033127-e1033127
07/03/2015
Handle:
https://hdl.handle.net/2376/108618
PMCID: PMC4622583
PMID: 26251877
Abstract
The symbiotic association between the legume Lotus japonicus and the nitrogen-fixing bacterium Mesorhizobium loti results in the formation of root nodules. This process begins with the recognition of the rhizobial nodulation factor (NF) by the NF receptors (NFR) at the cell surface of the host roots. The downstream signaling cascades after NFR recognition have not been fully characterized. We recently identified a clathrin heavy chain 1 (CHC1) from L. japonicus as a potential target of the NF signaling cascades. CHC is a known central component in the clathrin-mediated endocytosis (CME) in eukaryotic cells. The CHC1 gene was highly expressed in Rhizobium-infected root hairs and the CHC1 protein was present in cytoplasmic punctate structures near the infection pockets and along the infection thread membrane. Furthermore, expression of a dominant-negative variant of CHC1 or treatment with a chemical inhibitor of CME resulted in impaired phenotypes in the NF signaling, rhizobial infection and nodulation. These findings open a new avenue for future work aiming at understanding the role of endocytosis in NF signaling pathway and rhizobial infection.
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Details
- Title
- Involvement of ROP6 and clathrin in nodulation factor signaling
- Creators
- Chao Wang - State Key Laboratory of Agricultural Microbiology; Huazhong Agricultural UniversityXiaoshu Xu - Shanghai Institutes for Biological SciencesZonglie Hong - Department of Plant, Soil and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry; University of IdahoYong Feng - State Key Laboratory of Agricultural Microbiology; Huazhong Agricultural UniversityZhongming Zhang - State Key Laboratory of Agricultural Microbiology; Huazhong Agricultural University
- Publication Details
- Plant signaling & behavior, Vol.10(7), pp.e1033127-e1033127
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Taylor & Francis
- Identifiers
- 99900547104901842
- Language
- English
- Resource Type
- Journal article