Involvement of a NO–cyclic GMP–PKG signaling pathway in nitrous oxide-induced antinociception in mice

Yao Zhang; Lindsay P Quock; Eunhee Chung; Yusuke Ohgami; Raymond M Quock

doi:10.1016/j.ejphar.2011.01.006

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Involvement of a NO–cyclic GMP–PKG signaling pathway in nitrous oxide-induced antinociception in mice

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Involvement of a NO–cyclic GMP–PKG signaling pathway in nitrous oxide-induced antinociception in mice

Yao Zhang, Lindsay P Quock, Eunhee Chung, Yusuke Ohgami and Raymond M Quock

European journal of pharmacology, Vol.654(3), pp.249-253

2011

DOI: https://doi.org/10.1016/j.ejphar.2011.01.006

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https://hdl.handle.net/2376/106264

PMCID: PMC3050811

PMID: 21238450

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Abstract

Antinociception

Nitrous oxide

Mouse

Nitric oxide

Cyclic GMP

Protein kinase G

The antinociceptive effect of nitrous oxide (N 2O) is dependent on nitric oxide (NO); however, the next step in the pathway activated by NO is undetermined. The present study was conducted to test the hypothesis that a N 2O action involves sequential activation of NO synthase, soluble guanylyl cyclase and protein kinase G to induce an antinociceptive effect in mice. The antinociceptive responsiveness of male NIH Swiss mice to N 2O was assessed using the acetic acid abdominal constriction test. Different groups of mice were pretreated with either saline, the NO scavenger 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazolyl-1-oxy-3-oxide (carboxy-PTIO), the guanylyl cyclase-inhibitor 1H-[1,2,4]-oxadiazolo-[4,3-a]quinoxalin-1-one (ODQ), the protein kinase G-inhibitor Rp-isomer of 8-(4-chlorophenylthio)-guanosine-3′,5′-cyclic monophosphorothioate (Rp-8-pCPT-cGMPS) or the selective phosphodiesterase V-inhibitor 1,2-dihydro-2-[(2-methyl-4-pyridinyl)methyl]-1-oxo-8-(2-pyrimidinylmethoxy)-4-(3,4,5-trimethoxyphenyl)-2,7-naphthyridine-3-carboxylic acid methyl ester hydrochloride (T 0156). Vehicle (saline)-pretreated mice responded to N 2O in a concentration-dependent manner. This antinociceptive effect was antagonized by systemic pretreatment with carboxy-PTIO and ODQ and central pretreatment with Rp-8-pCPT-cGMPS. In each case, the dose–response curve for N 2O was progressively shifted to the right by increasing the dose of each pretreatment drug. On the other hand, N 2O-induced antinociception was enhanced by systemic pretreatment with T 0156; the dose–response curve for N 2O was shifted to the left. The ATP-sensitive potassium channel blocker glibenclamide was without influence on the antinociceptive effect of N 2O. These results support the hypothesis that N 2O-induced antinociception in mice is mediated by a NO–cyclic GMP–PKG pathway. [Display omitted]

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Title: Involvement of a NO–cyclic GMP–PKG signaling pathway in nitrous oxide-induced antinociception in mice
Creators: Yao Zhang - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164-6534, USA
Lindsay P Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164-6534, USA
Eunhee Chung - Institute of Scientific Criminal Investigation, Graduate School of Peace and Security, Chungnam National University, Daejeon 305-764, South Korea
Yusuke Ohgami - Department of Scientific Criminal Investigation, Graduate School of Peace and Security, Chungnam National University, Daejeon 305-764, South Korea
Raymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA 99164-6534, USA
Publication Details: European journal of pharmacology, Vol.654(3), pp.249-253
Academic Unit: Psychology, Department of
Publisher: Elsevier B.V
Identifiers: 99900546710501842
Language: English
Resource Type: Journal article