Journal article
Involvement of bovine leukemia virus in induction and inhibition of apoptosis
Microbes and infection, Vol.7(1), pp.19-28
2005
Handle:
https://hdl.handle.net/2376/103464
PMID: 15716078
Abstract
In a previous study, we identified an interesting mutant form of the Tax protein of bovine leukemia virus (BLV), designated D247G, that has an enhanced capacity to transactivate the long terminal repeat (LTR) of BLV and the cellular proto-oncogene
c-fos when compared with wild-type Tax (wt-Tax). We demonstrate here that an infectious strain of BLV containing the mutant D247G form of Tax also differs in its capacity to modulate cell survival both positively and negatively. When peripheral blood mononuclear cells (PBMCs) infected with wild-type or mutant BLV are cultured ex vivo with staurosporine, an agent known to induce a mitochondrial caspase cascade pathway regulating apoptosis, the rate of apoptosis is reduced to a greater extent in cells infected with mutant BLV than wild-type BLV, consistent with previous observations in cultures without staurosporine. The increase in survival was associated with an increase in expression of mRNA of
bcl-xl but not
bcl-2 and
bax ex vivo. In contrast, when a tissue culture-adapted cell line, 293T, was transiently transfected with either wild-type or mutant BLV, apoptosis was induced. The increase in the rate of apoptosis was higher in cells transfected with mutant BLV. The same difference was noted in cells transiently transfected with wild-type and mutant D247G Tax, suggesting that the observed positive and negative modulation of cell survival is attributed to the functional characteristics of mutant D247G Tax.
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Details
- Title
- Involvement of bovine leukemia virus in induction and inhibition of apoptosis
- Creators
- Masahiko Takahashi - Retrovirus Research Unit, RIKEN, Wako, Saitama 351-0198, JapanShigeru Tajima - Retrovirus Research Unit, RIKEN, Wako, Saitama 351-0198, JapanKosuke Okada - Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550, JapanWilliam C Davis - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USAYoko Aida - Retrovirus Research Unit, RIKEN, Wako, Saitama 351-0198, Japan
- Publication Details
- Microbes and infection, Vol.7(1), pp.19-28
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier SAS
- Identifiers
- 99900546797201842
- Language
- English
- Resource Type
- Journal article