Journal article
Involvement of brain opioid receptors in the anti-allodynic effect of hyperbaric oxygen in rats with sciatic nerve crush-induced neuropathic pain
Brain research, Vol.1537, pp.111-116
11/06/2013
Handle:
https://hdl.handle.net/2376/106695
PMCID: PMC3827781
PMID: 23998986
Abstract
Earlier research has demonstrated that hyperbaric oxygen (HBO2) can produce an antinociceptive effect in models of acute pain. Recent studies have revealed that HBO2 can produce pain relief in animal models of chronic pain as well. The purpose of the present investigation was to ascertain whether HBO2 treatment might suppress allodynia in rats with neuropathic pain and whether this effect might be blocked by the opioid antagonist naltrexone (NTX). Male Sprague Dawley rats were subjected to a sciatic nerve crush under anesthesia and mechanical thresholds were assessed using an electronic von Frey anesthesiometer. The time course of the HBO2-induced anti-allodynic effect in different treatment groups was plotted, and the area-under-the-curve (AUC) was determined for each group. Seven days after the nerve crush procedure, rats were treated with HBO2 at 3.5atm absolute (ATA) for 60min and exhibited an anti-allodynic effect, compared to nerve crush-only control rats. Twenty-four hours before HBO2 treatment, another group of rats was implanted with Alzet® osmotic minipumps that continuously released NTX into the lateral cerebral ventricle for 7 days. These NTX-infused, HBO2-treated rats exhibited an allodynic response comparable to that exhibited by rats receiving nerve crush only. Analysis of the AUC data showed that HBO2 significantly reduced the nerve crush-induced allodynia; this anti-allodynic effect of HBO2 was reversed by NTX. These results implicate opioid receptors in the pain relief induced by HBO2.
•Sciatic nerve crush produces a state of allodynia that lasts for up to 29 days after the procedure.•HBO2 treatment seven days after nerve crush produces an antiallodynic effect.•Implantation of an Alzet® osmotic minipump releasing 1.0μg/h of naltrexone reversed the HBO2-induced antiallodynia.
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Details
- Title
- Involvement of brain opioid receptors in the anti-allodynic effect of hyperbaric oxygen in rats with sciatic nerve crush-induced neuropathic pain
- Creators
- Carlee R Gibbons - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USAShulin Liu - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USAYangmiao Zhang - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USACasey L Sayre - Faculty of Pharmacy, University of Manitoba, Winnipeg, MB, CanadaBriana R Levitch - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USASarah B Moehlmann - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USADonald Y Shirachi - Department of Physiology and Pharmacology, Thomas J. Long School of Pharmacy and Health Sciences, University of the Pacific, Stockton, CA, USARaymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USA
- Publication Details
- Brain research, Vol.1537, pp.111-116
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546897001842
- Language
- English
- Resource Type
- Journal article