Journal article
Involvement of dsRNA virus in the protein composition and growth kinetics of host Trichomonas vaginalis
Archives of virology, Vol.142(5), pp.939-952
1997
Handle:
https://hdl.handle.net/2376/107350
PMID: 9191859
Abstract
Trichomonas vaginalis harbors a double-stranded (ds)-RNA virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (Khoshnan A, Alderete JF (1994) J Virol 68: 4035-4038). To further test the influence of virus on T. vaginalis, virus-infected (V+) isolates were compared to virus-free (V-), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. Comparative high resolution two dimensional (2D)-SDS-PAGE was performed of trichomonads grown in a chemostat under identical conditions. At least 47 proteins were identified as specifically expressed by representative V+ isolate 347, and approximately 41 spots were specific to the corresponding V- progeny, showing an association between virus and the presence and absence of parasite proteins. Qualitatively and quantitatively dissimilar cysteine proteinase patterns were detected from numerous V+ isolates and the V- progeny. A 2D analysis for isolate 347 showed the appearance of unique proteinase activities for parental parasites and presence of at least one proteinase in the V- progeny. Finally, the V+ T. vaginalis isolate 347, but not the V- isolate 347 progeny nor other V+ isolates, underwent fluctuations in density during chemostat growth allowing for purification of virus particles from the V+ isolate 347 supernatants during decreased parasite density.
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Details
- Title
- Involvement of dsRNA virus in the protein composition and growth kinetics of host Trichomonas vaginalis
- Creators
- D Provenzano - Department of Microbiology, University of Texas Health Science Center, San Antonio, USAA KhoshnanJ F Alderete
- Publication Details
- Archives of virology, Vol.142(5), pp.939-952
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Austria
- Grant note
- AI 39803 / NIAID NIH HHS AI 18768 / NIAID NIH HHS
- Identifiers
- 99900546960101842
- Language
- English
- Resource Type
- Journal article