Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions

Maolei Zhu; Brad Bendiak; Brian Clowers; Herbert H Hill, Jr

doi:10.1007/s00216-009-2865-y

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Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions

Journal article

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Peer reviewed

Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions

Maolei Zhu, Brad Bendiak, Brian Clowers and Herbert H Hill, Jr

Analytical and bioanalytical chemistry, Vol.394(7), pp.1853-1867

08/2009

DOI: https://doi.org/10.1007/s00216-009-2865-y

Handle:

https://hdl.handle.net/2376/106678

PMID: 19562326

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Abstract

Animals

Time Factors

Cattle

Spectrometry, Mass, Electrospray Ionization - methods

Oligosaccharides - analysis

Ions - analysis

Isomerism

Spectrometry, Mass, Electrospray Ionization - instrumentation

The rapid separation of isomeric precursor ions of oligosaccharides prior to their analysis by mass spectrometry to the nth power (MS(n)) was demonstrated using an ambient pressure ion mobility spectrometer (IMS) interfaced with a quadrupole ion trap. Separations were not limited to specific types of isomers; representative isomers differing solely in the stereochemistry of sugars, in their anomeric configurations, and in their overall branching patterns and linkage positions could be resolved in the millisecond time frame. Physical separation of precursor ions permitted independent mass spectra of individual oligosaccharide isomers to be acquired to at least MS(3), the number of stages of dissociation limited only practically by the abundance of specific product ions. IMS-MS(n) analysis was particularly valuable in the evaluation of isomeric oligosaccharides that yielded identical sets of product ions in tandem mass spectrometry experiments, revealing pairs of isomers that would otherwise not be known to be present in a mixture if evaluated solely by MS dissociation methods alone. A practical example of IMS-MS(n) analysis of a set of isomers included within a single high-performance liquid chromatography fraction of oligosaccharides released from bovine submaxillary mucin is described.

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Title: Ion mobility-mass spectrometry analysis of isomeric carbohydrate precursor ions
Creators: Maolei Zhu - Department of Chemistry, Washington State University, Pullman, WA 99163, USA
Brad Bendiak
Brian Clowers
Herbert H Hill, Jr
Publication Details: Analytical and bioanalytical chemistry, Vol.394(7), pp.1853-1867
Academic Unit: Chemistry, Department of
Publisher: Germany
Grant note: R33 RR020046-04 / NCRR NIH HHS R33 RR020046-05 / NCRR NIH HHS R33 RR020046-03 / NCRR NIH HHS 5R21RR02004602 / NCRR NIH HHS
Identifiers: 99900546844901842
Language: English
Resource Type: Journal article