Journal article
Iron modulates phenotypic variation and phosphorylation of P270 in double-stranded RNA virus-infected Trichomonas vaginalis
Infection and immunity, Vol.67(8), pp.4298-4302
08/1999
Handle:
https://hdl.handle.net/2376/110086
PMCID: PMC96743
PMID: 10417210
Abstract
Trichomonas vaginalis infected with a double-stranded RNA virus undergoes phenotypic variation on the basis of surface versus cytoplasmic expression of the immunogenic protein P270. Examination of batch cultures by flow cytofluorometry with monoclonal antibody (MAb) to P270 yields both fluorescent and nonfluorescent trichomonads. Greater numbers and intensity of fluorescent organisms with surface P270 reactive with MAb were evident in parasites grown in medium depleted of iron. Placement of iron-limited organisms in medium supplemented with iron gave increased numbers of nonfluorescent trichomonads. Purified subpopulations of trichomonads with and without surface P270 obtained by fluorescence-activated cell sorting reverted to nonfluorescent and fluorescent phenotypes when placed in high- and low-iron media, respectively. No similar regulation by iron of P270 was evident among virus-negative T. vaginalis isolates or virus-negative progeny trichomonads derived from virus-infected isolates. Equal amounts of P270 were detectable by MAb on immunoblots of total proteins from identical numbers of parasites grown in low- and high-iron media. Finally, P270 was found to be highly phosphorylated in high-iron parasites. Iron, therefore, plays a role in modulating surface localization of P270 in virus-harboring parasites.
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Details
- Title
- Iron modulates phenotypic variation and phosphorylation of P270 in double-stranded RNA virus-infected Trichomonas vaginalis
- Creators
- J F Alderete - Department of Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA. alderete@uthscsa.edu
- Publication Details
- Infection and immunity, Vol.67(8), pp.4298-4302
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- R21 AI043940 / NIAID NIH HHS AI-39803 / NIAID NIH HHS AI-43940 / NIAID NIH HHS R01 AI043940 / NIAID NIH HHS
- Identifiers
- 99900547295201842
- Language
- English
- Resource Type
- Journal article