Journal article
Isolate-specific parasite antigens of the Babesia bovis-infected erythrocyte surface
Molecular and biochemical parasitology, Vol.60(1), pp.121-132
1993
Handle:
https://hdl.handle.net/2376/105851
PMID: 8366886
Abstract
Bovine erythrocytes taken from in vitro cultures of
Babesia bovis parasites from Mexico and the United States were assayed for the presence of new epitopes on the erythrocyte surface. New surface-exposed epitopes were detected by means of a whole-cell antigen capture assay. These epitopes were subsequently demonstrated only on infected erythrocytes by immunofluorescence staining of intact, living cells. Parasite-synthesized antigens were identified on each isolate using a surface-specific immunoprecipitation technique to analyze metabolically-labeled infected erythrocytes. In the Mexico isolate these antigens were 120 kDa and 107 kDa, whereas in the United States isolate polypeptides of 135, 120 and 107 kDa were detected. In each of these assays, reaction of immune sera with the infected erythrocyte surface was found to be isolate-specific.
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Details
- Title
- Isolate-specific parasite antigens of the Babesia bovis-infected erythrocyte surface
- Creators
- David R Allred - Department of Infectious Diseases, University of Florida, Gainesville, FL, USAStephen A Hines - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USAKim P Ahrens - Department of Infectious Diseases, University of Florida, Gainesville, FL, USA
- Publication Details
- Molecular and biochemical parasitology, Vol.60(1), pp.121-132
- Academic Unit
- Veterinary Medicine, College of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546856001842
- Language
- English
- Resource Type
- Journal article