Journal article
Isolation and characterization of an NAD+-degrading bacterium PTX1 and its role in chromium biogeochemical cycle
Biodegradation, Vol.19(3), pp.417-424
06/2008
Handle:
https://hdl.handle.net/2376/115029
PMID: 17701280
Abstract
Microorganisms can reduce toxic chromate to less toxic trivalent chromium [Cr(III)]. Besides Cr(OH)3 precipitates, some soluble organo-Cr(III) complexes are readily formed upon microbial, enzymatic, and chemical reduction of chromate. However, the biotransformation of the organo-Cr(III) complexes has not been characterized. We have previously reported the formation of a nicotinamide adenine dinucleotide (NAD+)-Cr(III) complex after enzymatic reduction of chromate. Although the NAD+-Cr(III) complex was stable under sterile conditions, microbial cells were identified as precipitates in a non-sterile NAD+-Cr(III) solution after extended incubation. The most dominant bacterium PTX1 was isolated and assigned to Leifsonia genus by phylogenetic analysis of 16S rRNA gene sequence. PTX1 grew slowly on NAD+ with a doubling time of 17 h, and even more slowly on the NAD+-Cr(III) complex with an estimated doubling time of 35 days. The slow growth suggests that PTX1 passively grew on trace NAD+ dissociated from the NAD+-Cr(III) complex, facilitating further dissociation of the complex and formation of Cr(III) precipitates. Thus, organo-Cr(III) complexes might be an intrinsic link of the chromium biogeochemical cycle; they can be produced during chromate reduction and then further mineralized by microorganisms.
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Details
- Title
- Isolation and characterization of an NAD+-degrading bacterium PTX1 and its role in chromium biogeochemical cycle
- Creators
- Geoffrey Puzon - School of Molecular Biosciences Washington State University Abelson Hall 301 Pullman WA 99164-4234 USAYan Huang - Washington State UniversityAlice Dohnalkova - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99352 USALuying Xun - School of Molecular Biosciences Washington State University Abelson Hall 301 Pullman WA 99164-4234 USA
- Publication Details
- Biodegradation, Vol.19(3), pp.417-424
- Academic Unit
- School of Molecular Biosciences
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547316701842
- Language
- English
- Resource Type
- Journal article