Journal article
Isolation, characterization, and validation of oleaginous, multi-trophic, and haloalkaline-tolerant microalgae for two-stage cultivation
Algal research (Amsterdam), Vol.4(1), pp.2-11
04/2014
Handle:
https://hdl.handle.net/2376/112231
Abstract
Mass outdoor cultivation of microalgae for biofuels and co-products faces challenges of low lipid productivity, contamination, inefficient CO2 supply, and difficulties in harvesting. A two-stage cultivation process was developed to address these challenges. This involves culturing microalgae in a fermentor heterotrophically or photobioreactor mixotrophically as first-stage to rapidly obtain high cell densities for inoculating a phototrophic open-pond culture featuring high levels of NaHCO3, pH, and salinity as second-stage. A microalgae that is tolerant of these phototrophic conditions, can use organic carbon, and can prolifically produce oil is key to the success of such a two-stage process. Two oleaginous, haloalkaline-tolerant, and multi-trophic green microalgae from soda lakes were isolated, identified, and compared in this study using a multi-instrument approach as candidates for such process. A model triacylglyceride (TAG) was developed for rapid, non-destructive lipid quantitation using liquid-state 1H NMR. A two-stage cultivation system and a high pH-mediated auto-flocculation method were tested on the selected strain ALP2 with a 1L fermentor and 40L open-tank. In unoptimized conditions, the stain achieved a final biomass concentration of 0.978gDCW/L, lipid content of 39.78% DCW, and auto-flocculation harvesting efficiency of 64.1%.
•We examine new oleaginous, multi-trophic, haloalkaline-tolerant microalgae.•We demonstrate a new heterotrophic-phototrophic two-stage cultivation strategy.•High NaHCO3 and pH facilitate mono-cultivation, harvesting, and carbon delivery.•We develop an 1H NMR method to detect neutral lipid in microalgal cultures.•We use flow cytometry to sort microalgal cells and monitor contamination.
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Details
- Title
- Isolation, characterization, and validation of oleaginous, multi-trophic, and haloalkaline-tolerant microalgae for two-stage cultivation
- Creators
- Pierre Wensel - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, United StatesGreg Helms - Center for NMR Spectroscopy, Washington State University, Pullman, WA 99164, United StatesBill Hiscox - Center for NMR Spectroscopy, Washington State University, Pullman, WA 99164, United StatesWilliam C Davis - College of Veterinary Medicine, Washington State University, Pullman, WA 99164, United StatesHelmut Kirchhoff - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164, United StatesMahesh Bule - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, United StatesLiang Yu - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, United StatesShulin Chen - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, United States
- Publication Details
- Algal research (Amsterdam), Vol.4(1), pp.2-11
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900583057001842
- Language
- English
- Resource Type
- Journal article