Journal article
Isolation of sockeye salmon growth hormone utilizing serum triiodothyronine enhancement in rainbow trout to monitor biological activity
General and comparative endocrinology, Vol.84(3), pp.374-380
1991
Handle:
https://hdl.handle.net/2376/105745
PMID: 1808018
Abstract
Growth hormone (GH) was isolated from sockeye salmon (
Oncorhynchus nerka) pituitary glands using established techniques of affinity and gel filtration chromatography, and preparative polyacrylamide gel electrophoresis. The GH activity was followed throughout the fractionation procedure with a bioassay based on the increase of serum triiodothyronine (T
3) in rainbow trout (
Oncorhynchus mykiss), measured by radioimmunoassay (RIA). Aminoterminal amino acid sequence analysis and subsequent comparison with established GH sequences from other
Oncorhynchus sp. were used to confirm the isolation of sockeye salmon GH (ssGH). The bioassay was sensitive to a dose of 55 ng of purified ssGH/g fish. Monomeric GHs, located in the carbohydrate-poor protein fraction, were the only pituitary components that elevated serum T
3. Twenty-four hours after GH injection was an appropriate and practical time to blood sample, allowing completion of the bioassay, including RIA, in 3 days. The generic homology, between the source of pituitaries and the bioassay animals used in this study, should permit the bioassay to be useful during GH isolation from pituitaries of all
Oncorhynchus sp.
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Details
- Title
- Isolation of sockeye salmon growth hormone utilizing serum triiodothyronine enhancement in rainbow trout to monitor biological activity
- Creators
- James J NaglerShinn Jia HwangDavid R Idler
- Publication Details
- General and comparative endocrinology, Vol.84(3), pp.374-380
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Inc
- Identifiers
- 99900547071401842
- Language
- English
- Resource Type
- Journal article