Journal article
Isolation of the GA-response mutant sly1 as a suppressor of ABI1-1 in Arabidopsis thaliana
Genetics (Austin), Vol.149(2), pp.509-521
06/1998
Handle:
https://hdl.handle.net/2376/112553
PMCID: PMC1460187
PMID: 9611170
Abstract
Seed dormancy and germination in higher plants are partially controlled by the plant hormones abscisic acid (ABA) and gibberellic acid (GA). ABA establishes dormancy during embryo maturation, whereas GA breaks dormancy and induces germination. Previous attempts to identify GA response genes were confounded because GA mutants are not expected to germinate and, unlike GA auxotrophs, should fail to be rescued by exogenous GA. Here, we describe a screen for suppressors of the ABA-insensitive mutant ABI1-1 that enriches for GA auxotrophs and GA-insensitive mutants. The vast majority (76%) of the suppressors of ABI1-1 strongly resemble GA auxotrophs in that they are severely dwarfed and have dark green foliage and flowers with underdeveloped petals and stamen. Three isolates were alleles of the GA auxotroph ga1. The remaining severe dwarves were not rescued by GA and belong to a single complementation group that we designate sly1 (Sleepy 1). The alleles of sly1 identified are the first recessive GA-insensitive mutations to reflect the full spectrum of GA-associated phenotypes, including the failure to germinate in the absence of the ABI1-1 lesion. Thus, we postulate that SLY1 is a key factor in GA reception.
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Details
- Title
- Isolation of the GA-response mutant sly1 as a suppressor of ABI1-1 in Arabidopsis thaliana
- Creators
- C M Steber - Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, CanadaS E Cooney - Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, CanadaP McCourt - Department of Botany, University of Toronto, Toronto, Ontario M5S 3B2, Canada
- Publication Details
- Genetics (Austin), Vol.149(2), pp.509-521
- Academic Unit
- Crop and Soil Sciences, Department of
- Identifiers
- 99900548011301842
- Language
- English
- Resource Type
- Journal article