Isotope dilution mass spectrometry for the quantification of sulfane sulfurs

Chunrong Liu; Faya Zhang; Gerhard Munske; Hui Zhang; Ming Xian

doi:10.1016/j.freeradbiomed.2014.08.003

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Isotope dilution mass spectrometry for the quantification of sulfane sulfurs

Journal article

Open access

Peer reviewed

Isotope dilution mass spectrometry for the quantification of sulfane sulfurs

Chunrong Liu, Faya Zhang, Gerhard Munske, Hui Zhang and Ming Xian

Free radical biology & medicine, Vol.76, pp.200-207

11/2014

DOI: https://doi.org/10.1016/j.freeradbiomed.2014.08.003

Handle:

https://hdl.handle.net/2376/113359

PMCID: PMC4254298

PMID: 25152234

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https://doi.org/10.1016/j.freeradbiomed.2014.08.003View

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Abstract

Tissue Distribution

Isotope Labeling - methods

Animals

Phosphines - chemistry

Hydrogen Sulfide - chemistry

Mice, Inbred C57BL

Sulfur Compounds - analysis

Female

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Mice

Sulfur Compounds - pharmacokinetics

Sulfane sulfurs are one type of important reactive sulfur species. These molecules have unique reactivity that allows them to attach reversibly to other sulfur atoms and exhibit regulatory effects in diverse biological systems. Recent studies have suggested that sulfane sulfurs are involved in signal transduction processes regulated by hydrogen sulfide (H2S). Accurate and reliable measurements of sulfane sulfurs in biological samples are thus needed to reveal their production and mechanisms of actions. Herein we report a convenient and accurate method for the determination of sulfane sulfur concentrations. The method employs a triphenylphosphine derivative (P2) to capture sulfane sulfurs as a stable phosphine sulfide product, PS2. The concentration of PS2 was then determined by isotope dilution mass spectrometry, using a (13)C3-labeled phosphine sulfide, PS1, as the internal standard. The specificity and efficiency of the method were proven by model reactions. It was also applied to the measurement of sulfane sulfurs in mouse tissues including brain, kidney, lung, liver, heart, spleen, and blood.

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Title: Isotope dilution mass spectrometry for the quantification of sulfane sulfurs
Creators: Chunrong Liu - Department of Chemistry, Washington State University, Pullman, WA 99164, USA; Research Institute of Science and Technology, Central China Normal University, Wuhan 430079, China
Faya Zhang - Department of Pharmaceutical Sciences, Washington State University, Pullman, WA 99164, USA
Gerhard Munske - Center for Reproductive Biology, Molecular Biology and Genomics Core, Washington State University, Pullman, WA 99164, USA
Hui Zhang - Department of Pharmaceutical Sciences, Washington State University, Pullman, WA 99164, USA
Ming Xian - Department of Chemistry, Washington State University, Pullman, WA 99164, USA. Electronic address: mxian@wsu.edu
Publication Details: Free radical biology & medicine, Vol.76, pp.200-207
Academic Unit: Chemistry, Department of; Pharmaceutical Sciences, Department of
Publisher: United States
Grant note: R01 HL116571 / NHLBI NIH HHS R01HL116571 / NHLBI NIH HHS
Identifiers: 99900548120601842
Language: English
Resource Type: Journal article