Journal article
Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize
Plant biotechnology journal, Vol.14(7), pp.1551-1562
07/2016
Handle:
https://hdl.handle.net/2376/106892
PMCID: PMC5066742
PMID: 26801971
Abstract
Both insufficient and excessive male inflorescence size leads to a reduction in maize yield. Knowledge of the genetic architecture of male inflorescence is essential to achieve the optimum inflorescence size for maize breeding. In this study, we used approximately eight thousand inbreds, including both linkage populations and association populations, to dissect the genetic architecture of male inflorescence. The linkage populations include 25 families developed in the U.S. and 11 families developed in China. Each family contains approximately 200 recombinant inbred lines (RILs). The association populations include approximately 1000 diverse lines from the U.S. and China. All inbreds were genotyped by either sequencing or microarray. Inflorescence size was measured as the tassel primary branch number (TBN) and tassel length (TL). A total of 125 quantitative trait loci (QTLs) were identified (63 for TBN, 62 for TL) through linkage analyses. In addition, 965 quantitative trait nucleotides (QTNs) were identified through genomewide study (GWAS) at a bootstrap posterior probability (BPP) above a 5% threshold. These QTLs/QTNs include 24 known genes that were cloned using mutants, for example Ramosa3 (ra3), Thick tassel dwarf1 (td1), tasselseed2 (ts2), liguleless2 (lg2), ramosa1 (ra1), barren stalk1 (ba1), branch silkless1 (bd1) and tasselseed6 (ts6). The newly identified genes encode a zinc transporter (e.g. GRMZM5G838098 and GRMZM2G047762), the adapt in terminal region protein (e.g. GRMZM5G885628), O-methyl-transferase (e.g. GRMZM2G147491), helix-loop-helix (HLH) DNA-binding proteins (e.g. GRMZM2G414252 and GRMZM2G042895) and an SBP-box protein (e.g. GRMZM2G058588). These results provide extensive genetic information to dissect the genetic architecture of inflorescence size for the improvement of maize yield.
Metrics
3 Record Views
Details
- Title
- Joint-linkage mapping and GWAS reveal extensive genetic loci that regulate male inflorescence size in maize
- Creators
- Xun Wu - Nanchong Academy of Agricultural Sciences, Nanchong, Sichuan, ChinaYongxiang Li - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaYunsu Shi - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaYanchun Song - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaDengfeng Zhang - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaChunhui Li - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaEdward S Buckler - USA Department of Agriculture-Agricultural Research Service, Ithaca, NY, USAYu Li - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaZhiwu Zhang - Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USATianyu Wang - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China
- Publication Details
- Plant biotechnology journal, Vol.14(7), pp.1551-1562
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- England
- Identifiers
- 99900546729801842
- Language
- English
- Resource Type
- Journal article