Juvenile hormone regulates vitellogenin gene expression through insulin-like peptide signaling pathway in the red flour beetle, Tribolium castaneum

Zhentao Sheng; Jingjing Xu; Hua Bai; Fang Zhu; Subba R Palli

doi:10.1074/jbc.M111.269845

Back

Juvenile hormone regulates vitellogenin gene expression through insulin-like peptide signaling pathway in the red flour beetle, Tribolium castaneum

Journal article

Open access

Peer reviewed

Juvenile hormone regulates vitellogenin gene expression through insulin-like peptide signaling pathway in the red flour beetle, Tribolium castaneum

Zhentao Sheng, Jingjing Xu, Hua Bai, Fang Zhu and Subba R Palli

The Journal of biological chemistry, Vol.286(49), pp.41924-41936

12/09/2011

DOI: https://doi.org/10.1074/jbc.M111.269845

Handle:

https://hdl.handle.net/2376/101769

PMCID: PMC3234905

PMID: 22002054

Files and links (1)

url

https://doi.org/10.1074/jbc.M111.269845View

Published (Version of record) Open

Abstract

Peptides - chemistry

Signal Transduction

Coleoptera

Juvenile Hormones - metabolism

Gene Expression Regulation

Gene Silencing

Molecular Sequence Data

DNA Primers - genetics

Insulin - metabolism

Animals

RNA Interference

Forkhead Transcription Factors - metabolism

Models, Biological

Base Sequence

Female

Proteins - chemistry

Proto-Oncogene Proteins c-akt - metabolism

Vitellogenins - biosynthesis

Our recent studies identified juvenile hormone (JH) and nutrition as the two key signals that regulate vitellogenin (Vg) gene expression in the red flour beetle, Tribolium castaneum. Juvenile hormone regulation of Vg synthesis has been known for a long time in several insects, but the mechanism of JH action is not known. Experiments were conducted to determine the mechanism of action of these two signals in regulation of Vg gene expression. Injection of bovine insulin or FOXO double-stranded RNA into the previtellogenic, starved, or JH-deficient female adults increased Vg mRNA and protein levels, thereby implicating the pivotal role for insulin-like peptide signaling in the regulation of Vg gene expression and possible cross-talk between JH and insulin-like peptide signaling pathways. Reduction in JH synthesis or its action by RNAi-mediated silencing of genes coding for acid methyltransferase or methoprene-tolerant decreased expression of genes coding for insulin-like peptides (ILPs) and influenced FOXO subcellular localization, resulting in the down-regulation of Vg gene expression. Furthermore, JH application to previtellogenic female beetles induced the expression of genes coding for ILP2 and ILP3, and induced Vg gene expression. FOXO protein expressed in baculovirus system binds to FOXO response element present in the Vg gene promoter. These data suggest that JH functions through insulin-like peptide signaling pathway to regulate Vg gene expression.

Metrics

14 Record Views

See more details

Details

Title: Juvenile hormone regulates vitellogenin gene expression through insulin-like peptide signaling pathway in the red flour beetle, Tribolium castaneum
Creators: Zhentao Sheng - Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546
Jingjing Xu - Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546
Hua Bai - Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546
Fang Zhu - Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546
Subba R Palli - Department of Entomology, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546. Electronic address: rpalli@uky.edu
Publication Details: The Journal of biological chemistry, Vol.286(49), pp.41924-41936
Publisher: United States
Grant note: GM070559-07 / NIGMS NIH HHS R01 GM070559 / NIGMS NIH HHS
Identifiers: 99900546676501842
Language: English
Resource Type: Journal article