Journal article
Kinetic modeling and analysis of a vesicle system for immunosensor development
Biosensors & bioelectronics, Vol.12(2), pp.135-144
1997
Handle:
https://hdl.handle.net/2376/108324
PMID: 9011024
Abstract
A novel mechanism is presented for immunosensor development that uses an immunological competition reaction in a vesicle system. This system consists of a suspension of reconstituted vesicles, channel agonist, protein linker to block the channels, voltage sensitive dye and analyte to be detected. In the proposed mechanism analyte serves a catalytic role as individual analytes competitively displace multiple channel linkers through association with one channel, dissociation and new associations with other channels. When one channel opens on a vesicle a permanent Nernst potential develops for that vesicle leading to fluorescence of voltage sensitive dyes. The time constant of the redistribution from linker-channel form to analyte-channel form is 0·92/
k
4 (
k
4 is the off-rate constant for the analyte-channel association) in the region of analyte concentrations less than 10
−9 M. Kinetic analyses show that several factors, including concentration of analyte or linker, number of channels per vesicle, on-rate or off-rate constant of the linker-channel and on-rate constant of analyte-channel complexes have significant effects on the minimum signal response time.
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Details
- Title
- Kinetic modeling and analysis of a vesicle system for immunosensor development
- Creators
- Yong-Yi Yu - Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USABernard J Van Wie - Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USAAlan R Koch - Department of Zoology, Washington State University, Pullman, WA 99164-2710, USADavid F Moffett - Department of Zoology, Washington State University, Pullman, WA 99164-2710, USAWilliam C Davis - Department of Veterinary, Microbiology and Pathology, Washington State University, Pullman, WA 99164-2710, USA
- Publication Details
- Biosensors & bioelectronics, Vol.12(2), pp.135-144
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547224701842
- Language
- English
- Resource Type
- Journal article