Journal article
Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation
Journal of biotechnology, Vol.166(4), pp.192-197
07/20/2013
Handle:
https://hdl.handle.net/2376/113015
PMID: 23702163
Abstract
•We present an effective method for designing and optimizing cellulase production.•The method combines mathematics and chemometrics by kinetic model and mass balance.•Cellulase production and productivity increased by using this model-aided method.
Reducing the production cost of cellulase as the key enzyme for cellulose hydrolysis to fermentable sugars remains a major challenge for biofuel production. Because of the complexity of cellulase production, kinetic modeling and mass balance calculation can be used as effective tools for process design and optimization. In this study, kinetic models for cell growth, substrate consumption and cellulase production in batch fermentation were developed, and then applied in fed-batch fermentation to enhance cellulase production. Inhibition effect of substrate was considered and a modified Luedeking–Piret model was developed for cellulase production and substrate consumption according to the growth characteristics of Trichoderma reesei. The model predictions fit well with the experimental data. Simulation results showed that higher initial substrate concentration led to decrease of cellulase production rate. Mass balance and kinetic simulation results were applied to determine the feeding strategy. Cellulase production and its corresponding productivity increased by 82.13% after employing the proper feeding strategy in fed-batch fermentation. This method combining mathematics and chemometrics by kinetic modeling and mass balance can not only improve cellulase fermentation process, but also help to better understand the cellulase fermentation process. The model development can also provide insight to other similar fermentation processes.
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Details
- Title
- Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation
- Creators
- Lijuan MaChen LiZhenhua YangWendi JiaDongyuan ZhangShulin Chen
- Publication Details
- Journal of biotechnology, Vol.166(4), pp.192-197
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548107301842
- Language
- English
- Resource Type
- Journal article