Journal article
Kremen1 regulates mechanosensory hair cell development in the mammalian cochlea and the zebrafish lateral line
Scientific reports, Vol.6(1), pp.31668-31668
08/23/2016
Handle:
https://hdl.handle.net/2376/110609
PMCID: PMC4994024
PMID: 27550540
Abstract
Here we present spatio-temporal localization of Kremen1, a transmembrane receptor, in the mammalian cochlea, and investigate its role in the formation of sensory organs in mammal and fish model organisms. We show that Kremen1 is expressed in prosensory cells during cochlear development and in supporting cells of the adult mouse cochlea. Based on this expression pattern, we investigated whether Kremen1 functions to modulate cell fate decisions in the prosensory domain of the developing cochlea. We used gain and loss-of-function experiments to show that Kremen1 is sufficient to bias cells towards supporting cell fate, and is implicated in suppression of hair cell formation. In addition to our findings in the mouse cochlea, we examined the effects of over expression and loss of Kremen1 in the zebrafish lateral line. In agreement with our mouse data, we show that over expression of Kremen1 has a negative effect on the number of mechanosensory cells that form in the zebrafish neuromasts, and that fish lacking Kremen1 protein develop more hair cells per neuromast compared to wild type fish. Collectively, these data support an inhibitory role for Kremen1 in hair cell fate specification.
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Details
- Title
- Kremen1 regulates mechanosensory hair cell development in the mammalian cochlea and the zebrafish lateral line
- Creators
- Joanna F Mulvaney - Biological Sciences, Sunnybrook Research Institute, 2075 Bayview Ave, Toronto, ON, M4N 3M5, CanadaCathrine Thompkins - College of Arts and Sciences and Department of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA, USATeppei Noda - Biological Sciences, Sunnybrook Research Institute, 2075 Bayview Ave, Toronto, ON, M4N 3M5, CanadaKoji Nishimura - Biological Sciences, Sunnybrook Research Institute, 2075 Bayview Ave, Toronto, ON, M4N 3M5, CanadaWilly W Sun - Biological Sciences, Sunnybrook Research Institute, 2075 Bayview Ave, Toronto, ON, M4N 3M5, CanadaShuh-Yow Lin - Department of Surgery, School of Medicine, University of California San Diego, La Jolla, CA, USAAllison Coffin - College of Arts and Sciences and Department of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA, USAAlain Dabdoub - Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada
- Publication Details
- Scientific reports, Vol.6(1), pp.31668-31668
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- England
- Identifiers
- 99900547290301842
- Language
- English
- Resource Type
- Journal article