Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas

Tao Yang; Junye Jiang; Marina Burlyaeva; Jinguo Hu; Clarice J Coyne; Shiv Kumar; Robert Redden; Xuelian Sun; Fang Wang; Jianwu Chang; Xiaopeng Hao; Jianping Guan; Xuxiao Zong

doi:10.1186/1471-2229-14-65

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Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas

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Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas

Tao Yang, Junye Jiang, Marina Burlyaeva, Jinguo Hu, Clarice J Coyne, Shiv Kumar, Robert Redden, Xuelian Sun, Fang Wang, Jianwu Chang, …

BMC plant biology, Vol.14(1), pp.65-65

03/17/2014

DOI: https://doi.org/10.1186/1471-2229-14-65

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https://hdl.handle.net/2376/115363

PMCID: PMC4003855

PMID: 24635905

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Abstract

Lathyrus - genetics

Polymorphism, Genetic - genetics

Fabaceae - genetics

Alleles

Microsatellite Repeats - genetics

Grasspea (Lathyrus sativus L., 2n = 14), a member of the family Leguminosae, holds great agronomic potential as grain and forage legume crop in the arid areas for its superb resilience to abiotic stresses such as drought, flood and salinity. The crop could not make much progress through conventional breeding in the past, and there are hardly any detailed molecular biology studies due to paucity of reliable molecular markers representative of the entire genome. Using the 454 FLX Titanium pyrosequencing technique, 651,827 simple sequence repeat (SSR) loci were identified and 50,144 nonredundant primer pairs were successfully designed, of which 288 were randomly selected for validation among 23 L. sativus and one L. cicera accessions of diverse provenance. 74 were polymorphic, 70 monomorphic, and 144 with no PCR product. The number of observed alleles ranged from two to five, the observed heterozygosity from 0 to 0.9545, and Shannon's information index ranged from 0.1013 to 1.0980, respectively. The dendrogram constructed by using unweighted pair group method with arithmetic mean (UPGMA) based on Nei's genetic distance, showed obvious distinctions and understandable relationships among the 24 accessions. The large number of SSR primer pairs developed in this study would make a significant contribution to genomics enabled improvement of grasspea.

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Title: Large-scale microsatellite development in grasspea (Lathyrus sativus L.), an orphan legume of the arid areas
Creators: Tao Yang
Junye Jiang
Marina Burlyaeva
Jinguo Hu
Clarice J Coyne
Shiv Kumar
Robert Redden
Xuelian Sun
Fang Wang
Jianwu Chang
Xiaopeng Hao
Jianping Guan
Xuxiao Zong - The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zongxuxiao@caas.cn
Publication Details: BMC plant biology, Vol.14(1), pp.65-65
Academic Unit: Agricultural, Human, and Natural Resource Sciences, College of; Crop and Soil Sciences, Department of
Publisher: England
Identifiers: 99900548006001842
Language: English
Resource Type: Journal article