Journal article
Large-scale production of enhancer trapping lines for rice functional genomics
Plant science (Limerick), Vol.167(2), pp.281-288
2004
Handle:
https://hdl.handle.net/2376/107263
Abstract
Using a rapid and efficient
Agrobacterium tumefaciens mediated rice transformation protocol, we had got about 100,000 independent enhancer trapping lines of
japonica rice variety Nipponbare. T
1 seeds had already been obtained from 60,000 lines. Regeneration of transgenic plants could be as short as 3 months starting from callus induction, thus the occurrence of somaclonal variation was reduced to a minimum level. The frequencies of hygromycin-resistant callus induction and plant regeneration were over 90 and 80%, respectively. PCR and Southern blot analysis showed that about 95% hygromycin resistant plants were transgenic. Southern blot and segregation analysis of T
1 seeds showed that about 40% transgenic plants contained single copy insertion, and the average copy number was 1.7. Histochemical assay of T
0 plants revealed expression patterns in root, leaf, embryo and endosperm of the reporter gene β-glucurodinase (GUS). This large-scale enhancer trapping pool provides a valuable tool for functional analysis of rice genome.
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Details
- Title
- Large-scale production of enhancer trapping lines for rice functional genomics
- Creators
- Yongzhi YangHao PengHongmei HuangJinxia WuShirong JiaDafang HuangTiegang Lu
- Publication Details
- Plant science (Limerick), Vol.167(2), pp.281-288
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900546743501842
- Language
- English
- Resource Type
- Journal article