Journal article
Larval Zebrafish Lateral Line as a Model for Acoustic Trauma
eNeuro, Vol.5(4), p.ENEURO.0206-18.2018
07/2018
Handle:
https://hdl.handle.net/2376/113928
PMID: 30225343
Abstract
Excessive noise exposure damages sensory hair cells, leading to permanent hearing loss. Zebrafish are a highly tractable model that have advanced our understanding of drug-induced hair cell death, yet no comparable model exists for noise exposure research. We demonstrate the utility of zebrafish as model to increase understanding of hair cell damage from acoustic trauma and develop protective therapies. We created an acoustic trauma system using underwater cavitation to stimulate lateral line hair cells. We found that acoustic stimulation resulted in exposure time- and intensity-dependent lateral line and saccular hair cell damage that is maximal at 48-72 h post-trauma. The number of TUNEL+ lateral line hair cells increased 72 h post-exposure, whereas no increase was observed in TUNEL+ supporting cells, demonstrating that acoustic stimulation causes hair cell-specific damage. Lateral line hair cells damaged by acoustic stimulation regenerate within 3 d, consistent with prior regeneration studies utilizing ototoxic drugs. Acoustic stimulation-induced hair cell damage is attenuated by pharmacological inhibition of protein synthesis or caspase activation, suggesting a requirement for translation and activation of apoptotic signaling cascades. Surviving hair cells exposed to acoustic stimulation showed signs of synaptopathy, consistent with mammalian studies. Finally, we demonstrate the feasibility of this platform to identify compounds that prevent acoustic trauma by screening a small redox library for protective compounds. Our data suggest that acoustic stimulation results in lateral line hair cell damage consistent with acoustic trauma research in mammals, providing a highly tractable model for high-throughput genetic and drug discovery studies.
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Details
- Title
- Larval Zebrafish Lateral Line as a Model for Acoustic Trauma
- Creators
- Phillip M Uribe - Department of Integrative Physiology and Neuroscience, Washington State University, Vancouver, WA 98686Beija K Villapando - College of Arts and Sciences, Washington State University, Vancouver, WA 98686Kristy J Lawton - College of Arts and Sciences, Washington State University, Vancouver, WA 98686Zecong Fang - Mechanical Engineering Department, Washington State University, Vancouver, WA 98686Dmitry Gritsenko - Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, Chicago, IL 60607Ashwin Bhandiwad - Department of Psychology, University of Washington, Seattle, WA 98195Joseph A Sisneros - Department of Psychology, University of Washington, Seattle, WA 98195Jie Xu - Department of Mechanical and Industrial Engineering, University of Illinois at Chicago, Chicago, IL 60607Allison B Coffin - College of Arts and Sciences, Washington State University, Vancouver, WA 98686
- Publication Details
- eNeuro, Vol.5(4), p.ENEURO.0206-18.2018
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- United States
- Grant note
- R21 DC015636 / NIDCD NIH HHS
- Identifiers
- 99900548043401842
- Language
- English
- Resource Type
- Journal article