Journal article
Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart
Physiological genomics, Vol.12(2), pp.129-138
01/15/2003
Handle:
https://hdl.handle.net/2376/106155
PMID: 12429864
Abstract
We present the first quantitative gene expression analysis of cardiac aging under conditions of sedentary and active lifestyles using high-density oligonucleotide arrays representing 11,904 cDNAs and expressed sequence tags (ESTs). With these data, we test the hypothesis that exercise attenuates the gene expression changes that normally occur in the aging heart. Male mice ( Mus domesticus) were sampled from the 16th generation of selective breeding for high voluntary exercise. For the selective breeding protocol, breeders were chosen based on the maximum number of wheel revolutions run on days 5 and 6 of a test at 8 wk of age. For the colony sampled herein, mice were housed individually over their entire lifetimes (from weaning) either with or without access to running wheels. The hearts of these two treatment groups (active and sedentary) were assayed at middle age (20 mo) and old age (33 mo). Genes significantly affected by age in the hearts of the sedentary population by at least a 50% expression change ( n = 137) were distributed across several major categories, including inflammatory response, stress response, signal transduction, and energy metabolism. Genes significantly affected by age in the active population were fewer ( n = 62). Of the 42 changes in gene expression that were common to both treatment groups, 32 (72%) displayed smaller fold changes as a result of exercise. Thus exercise offset many age-related gene expression changes observed in the hearts of the sedentary animals. These results suggest that adaptive physiological mechanisms that are induced by exercise can retard many effects of aging on heart muscle at the transcriptional level.
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Details
- Title
- Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart
- Creators
- A. M Bronikowski - Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, Department of Zoology, University of Wisconsin, Madison, Wisconsin 53706P. A Carter - School of Biological Sciences, Washington State University, Pullman, Washington 99164T. J Morgan - School of Biological Sciences, Washington State University, Pullman, Washington 99164T Garland - Department of Biology, University of California, Riverside, California 92521N Ung - Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011T. D Pugh - Geriatric Research, Education and Clinical Center, W. S. Middleton Memorial Veterans Administration Hospital, Madison 53706R Weindruch - Geriatric Research, Education and Clinical Center, W. S. Middleton Memorial Veterans Administration Hospital, Madison 53706, Department of Medicine, University of Wisconsin, Madison, Wisconsin 53706T. A Prolla - Department of Genetics and Medical Genetics, University of Wisconsin, Madison, Wisconsin 53706
- Publication Details
- Physiological genomics, Vol.12(2), pp.129-138
- Academic Unit
- Biological Sciences, School of
- Identifiers
- 99900546714901842
- Language
- English
- Resource Type
- Journal article