Journal article
Limitations to photosynthesis by proton motive force-induced photosystem II photodamage
eLife, Vol.5
10/04/2016
Handle:
https://hdl.handle.net/2376/117472
PMCID: PMC5050024
PMID: 27697149
Abstract
The thylakoid proton motive force (
pmf
) generated during photosynthesis is the essential driving force for ATP production; it is also a central regulator of light capture and electron transfer. We investigated the effects of elevated
pmf
on photosynthesis in a library of
Arabidopsis thaliana
mutants with altered rates of thylakoid lumen proton efflux, leading to a range of steady-state
pmf
extents. We observed the expected
pmf-
dependent alterations in photosynthetic regulation, but also strong effects on the rate of photosystem II (PSII) photodamage. Detailed analyses indicate this effect is related to an elevated electric field (Δ
ψ
) component of the
pmf
, rather than lumen acidification, which
in vivo
increased PSII charge recombination rates, producing singlet oxygen and subsequent photodamage. The effects are seen even in wild type plants, especially under fluctuating illumination, suggesting that Δ
ψ
-induced photodamage represents a previously unrecognized limiting factor for plant productivity under dynamic environmental conditions seen in the field.
DOI:
http://dx.doi.org/10.7554/eLife.16921.001
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Details
- Title
- Limitations to photosynthesis by proton motive force-induced photosystem II photodamage
- Creators
- Geoffry A Davis - ,Atsuko Kanazawa - ,Mark Aurel Schöttler - ,Kaori Kohzuma - ,John E Froehlich - ,A William Rutherford - ,Mio Satoh-Cruz - ,Deepika Minhas - ,Stefanie Tietz - ,Amit Dhingra - ,David M Kramer - ,
- Publication Details
- eLife, Vol.5
- Academic Unit
- Horticulture, Department of
- Publisher
- eLife Sciences Publications, Ltd
- Grant note
- ; DE-FG02-91ER20021 / ; Wolfson Research Merit Award / ; BB/K002627/1 / ;
- Identifiers
- 99900547976101842
- Language
- English
- Resource Type
- Journal article