Journal article
Limited nucleosome migration can completely randomize DNA repair patches in intact human cells
Journal of molecular biology, Vol.196(2), pp.433-436
1987
Handle:
https://hdl.handle.net/2376/109803
PMID: 3656454
Abstract
Following irradiation of human cells with ultraviolet light, DNA repair patches are initially inserted near the 5′ and 3′ ends of nucleosome core DNA leaving a “gap” in repair synthesis (of ~ 50 bases) near the center of the core DNA. With time, however, these same repair patches become randomized, apparently by nucleosome migration. We have developed both an analytical expression and a computer algorithm (which simulates nucleosome migration along DNA) to determine the average distance nucleosomes must migrate to change the initial, non-uniform distribution of repair patches in nucleosomes to a random distribution. Both of these methods yielded the same result: nucleosomes must migrate an average of about 50 base-pairs in either direction to produce the randomization observed.
Metrics
8 Record Views
Details
- Title
- Limited nucleosome migration can completely randomize DNA repair patches in intact human cells
- Creators
- Gregory E ArnoldA.Keith DunkerMichael J Smerdon
- Publication Details
- Journal of molecular biology, Vol.196(2), pp.433-436
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547210201842
- Language
- English
- Resource Type
- Journal article