Limited transcriptional response of ovine microglia to prion accumulation

James B Stanton; Donald P Knowles; Douglas R Call; Bruce A Mathison; Timothy V Baszler

doi:10.1016/j.bbrc.2009.06.030

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Limited transcriptional response of ovine microglia to prion accumulation

Journal article

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Peer reviewed

Limited transcriptional response of ovine microglia to prion accumulation

James B Stanton, Donald P Knowles, Douglas R Call, Bruce A Mathison and Timothy V Baszler

Biochemical and biophysical research communications, Vol.386(2), pp.345-350

2009

DOI: https://doi.org/10.1016/j.bbrc.2009.06.030

Handle:

https://hdl.handle.net/2376/114362

PMCID: PMC2746661

PMID: 19523453

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Abstract

Ovine

Prion

Scrapie

Microarray

Microglia

The conversion of normal cellular prion protein to disease-associated prion protein (PrP Sc) is a fundamental component of prion disease pathogenesis. The molecular mechanisms contributing to prion conversion and the impact of PrP Sc accumulation on cellular biology are not fully understood. To further define the molecular changes associated with PrP Sc accumulation in cultured cells, the transcriptional profile of PrP Sc-accumulating primary ovine microglia was compared to the profile of PrP Sc-lacking microglia using the Affymetrix Bovine Genome Array. The experimental design included three biological replicates, each with three technical replicates, and samples that were collected at the point of near maximal PrP Sc accumulation levels as measured by ELISA. The array analysis revealed only 19 upregulated genes and 30 downregulated genes in PrP Sc-accumulating microglia. The results support the hypothesis that chronic PrP Sc accumulation in cultured microglia results in a limited transcriptional response.

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Title: Limited transcriptional response of ovine microglia to prion accumulation
Creators: James B Stanton - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
Donald P Knowles - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
Douglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
Bruce A Mathison - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
Timothy V Baszler - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
Publication Details: Biochemical and biophysical research communications, Vol.386(2), pp.345-350
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: Elsevier Inc
Identifiers: 99900547985601842
Language: English
Resource Type: Journal article