Journal article
Lipidomic and proteomic analysis of Caenorhabditis elegans lipid droplets and identification of ACS-4 as a lipid droplet-associated protein
Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol.1851(10), pp.1337-1345
10/2015
Handle:
https://hdl.handle.net/2376/108766
PMID: 26121959
Abstract
Lipid droplets are cytoplasmic organelles that store neutral lipids for membrane synthesis and energy reserves. In this study, we characterized the lipid and protein composition of purified Caenorhabditis elegans lipid droplets. These lipid droplets are composed mainly of triacylglycerols, surrounded by a phospholipid monolayer composed primarily of phosphatidylcholine and phosphatidylethanolamine. The fatty acid composition of the triacylglycerols is rich in fatty acid species obtained from the dietary Escherichia coli, including cyclopropane fatty acids and cis-vaccenic acid. Unlike other organisms, C. elegans lipid droplets contain very little cholesterol or cholesterol esters. Comparison of the lipid droplet proteomes of wild type and high-fat daf-2 mutant strains shows a very similar proteome in both strains, except that the most abundant protein in the C. elegans lipid droplet proteome, MDT-28, is relatively less abundant in lipid droplets isolated from daf-2 mutants. Functional analysis of lipid droplet proteins identified in our proteomic studies indicated an enrichment of proteins required for growth and fat homeostasis in C. elegans. Finally, we confirmed the localization of one of the newly identified lipid droplet proteins, ACS-4. We found that ACS-4 localizes to the surface of lipid droplets in the C. elegans intestine and skin. This study bolsters C. elegans as a model to study the dynamics and functions of lipid droplets in a multicellular organism.
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•We analyzed the lipid and protein composition of Caenorhabditis elegans lipid droplets.•Caenorhabditis elegans lipid droplets contain TAGs and very little cholesterol.•The major lipid droplet protein, MDT-28, is less abundant in daf-2 mutants.•LD associated proteins are enriched for proteins that influence fat accumulation.•We created an ACS-4::GFP transgenic strain and show localization on lipid droplets.
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Details
- Title
- Lipidomic and proteomic analysis of Caenorhabditis elegans lipid droplets and identification of ACS-4 as a lipid droplet-associated protein
- Creators
- Tracy L Vrablik - School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USAVladislav A Petyuk - Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USAEmily M Larson - School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USARichard D Smith - Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USAJennifer L Watts - School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Biochimica et biophysica acta. Molecular and cell biology of lipids, Vol.1851(10), pp.1337-1345
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546912201842
- Language
- English
- Resource Type
- Journal article