Journal article
Low pH-Induced Conformational Change in Herpes Simplex Virus Glycoprotein B
Journal of virology, Vol.84(8), pp.3759-3766
04/2010
Handle:
https://hdl.handle.net/2376/103896
PMCID: PMC2849479
PMID: 20147407
Abstract
Herpesviruses can enter host cells using pH-dependent endocytosis pathways in a cell-specific manner. Envelope glycoprotein B (gB) is conserved among all herpesviruses and is a critical component of the complex that mediates membrane fusion and entry. Here we demonstrate that mildly acidic pH triggers specific conformational changes in herpes simplex virus (HSV) gB. The antigenic structure of gB was specifically altered by exposure to low pH both
in vitro
and during entry into host cells. The oligomeric conformation of gB was altered at a similar pH range. Exposure to acid pH appeared to convert virion gB into a lower-order oligomer. The detected conformational changes were reversible, similar to those in other class III fusion proteins. Exposure of purified, recombinant gB to mildly acidic pH resulted in similar changes in conformation and caused gB to become more hydrophobic, suggesting that low pH directly affects gB. We propose that intracellular low pH induces alterations in gB conformation that, together with additional triggers such as receptor binding, are essential for virion-cell fusion during herpesviral entry by endocytosis.
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Details
- Title
- Low pH-Induced Conformational Change in Herpes Simplex Virus Glycoprotein B
- Creators
- Stephen J Dollery - Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 East Marshall Street, P.O. Box 980678, Richmond, Virginia 23298-0678Mark G Delboy - Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 East Marshall Street, P.O. Box 980678, Richmond, Virginia 23298-0678Anthony V Nicola - Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 East Marshall Street, P.O. Box 980678, Richmond, Virginia 23298-0678
- Publication Details
- Journal of virology, Vol.84(8), pp.3759-3766
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546567401842
- Language
- English
- Resource Type
- Journal article