Journal article
Luteinizing Hormone Receptor Activation in Ovarian Granulosa Cells Promotes Protein Kinase A-Dependent Dephosphorylation of Microtubule-Associated Protein 2D
Molecular endocrinology (Baltimore, Md.), Vol.22(7), pp.1695-1710
07/01/2008
Handle:
https://hdl.handle.net/2376/106871
PMCID: PMC2453602
PMID: 18467524
Abstract
Abstract
The actions of LH to induce ovulation and luteinization of preovulatory follicles are mediated principally by activation of cAMP-dependent protein kinase (PKA) in granulosa cells. PKA activity is targeted to specific locations in many cells by A kinase-anchoring proteins (AKAPs). We previously showed that FSH induces expression of microtubule-associated protein (MAP) 2D, an 80-kDa AKAP, in rat granulosa cells, and that MAP2D coimmunoprecipitates with PKA-regulatory subunits in these cells. Here we report a rapid and targeted dephosphorylation of MAP2D at Thr256/Thr259 after treatment with human chorionic gonadotropin, an LH receptor agonist. This event is mimicked by treatment with forskolin or a cAMP analog and is blocked by the PKA inhibitor myristoylated-PKI, indicating a role for cAMP and PKA signaling in phosphoregulation of granulosa cell MAP2D. Furthermore, we show that Thr256/Thr259 dephosphorylation is blocked by the protein phosphatase 2A (PP2A) inhibitor, okadaic acid, and demonstrate interactions between MAP2D and PP2A by coimmunoprecipitation and microcystin-agarose pull-down. We also show that MAP2D interacts with glycogen synthase kinase (GSK) 3β and is phosphorylated at Thr256/Thr259 by this kinase in the basal state. Increased phosphorylation of GSK3β at Ser9 and the PP2A B56δ subunit at Ser566 is observed after treatment with human chorionic gonadotropin and appears to result in LH receptor-mediated inhibition of GSK3β and activation of PP2A, respectively. Taken together, these results show that the phosphorylation status of the AKAP MAP2D is acutely regulated by LH receptor-mediated modulation of kinase and phosphatase activities via PKA.
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Details
- Title
- Luteinizing Hormone Receptor Activation in Ovarian Granulosa Cells Promotes Protein Kinase A-Dependent Dephosphorylation of Microtubule-Associated Protein 2D
- Creators
- Maxfield P Flynn - 1Department of Cell and Molecular Biology (M.P.F., E.T.M., A.B.K., M.H.-D.), Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611Evelyn T Maizels - 1Department of Cell and Molecular Biology (M.P.F., E.T.M., A.B.K., M.H.-D.), Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611Amelia B Karlsson - 1Department of Cell and Molecular Biology (M.P.F., E.T.M., A.B.K., M.H.-D.), Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611Thomas McAvoy - 2Laboratory of Molecular and Cellular Neuroscience (T.M., J.-H.A., A.C.N.), The Rockefeller University, New York, New York 10021Jung-Hyuck Ahn - 2Laboratory of Molecular and Cellular Neuroscience (T.M., J.-H.A., A.C.N.), The Rockefeller University, New York, New York 10021Angus C Nairn - 3Department of Psychiatry (A.C.N.), Yale University School of Medicine, New Haven, Connecticut 06508Mary Hunzicker-Dunn - 1Department of Cell and Molecular Biology (M.P.F., E.T.M., A.B.K., M.H.-D.), Northwestern University, Feinberg School of Medicine, Chicago, Illinois 60611
- Publication Details
- Molecular endocrinology (Baltimore, Md.), Vol.22(7), pp.1695-1710
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Oxford University Press
- Identifiers
- 99900546666901842
- Language
- English
- Resource Type
- Journal article