Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge

Darrilyn G Fraser; Steve R Leib; Bao Shan Zhang; Robert H Mealey; Wendy C Brown; Travis C McGuire

doi:10.1128/CDLI.12.8.983-993.2005

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Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge

Journal article

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Peer reviewed

Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge

Darrilyn G Fraser, Steve R Leib, Bao Shan Zhang, Robert H Mealey, Wendy C Brown and Travis C McGuire

Clinical and diagnostic laboratory immunology, Vol.12(8), pp.983-993

08/2005

DOI: https://doi.org/10.1128/CDLI.12.8.983-993.2005

Handle:

https://hdl.handle.net/2376/105752

PMCID: PMC1182189

PMID: 16085917

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https://doi.org/10.1128/CDLI.12.8.983-993.2005View

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Abstract

T-Lymphocyte Subsets - immunology

T-Lymphocytes, Cytotoxic - immunology

Amino Acid Sequence

Cell Proliferation

T-Lymphocyte Subsets - cytology

Peptides - immunology

Peptides - genetics

Molecular Sequence Data

Infectious Anemia Virus, Equine - immunology

Viral Load

Equine Infectious Anemia - virology

Infectious Anemia Virus, Equine - pathogenicity

Lymphocyte Activation - immunology

Animals

T-Lymphocytes, Helper-Inducer - immunology

Treatment Failure

T-Lymphocytes, Helper-Inducer - cytology

Horses

Equine Infectious Anemia - immunology

Epitopes, T-Lymphocyte - immunology

Lipoproteins - immunology

Viral Vaccines - immunology

The effect of immunization with five lipopeptides, three containing T-helper (Th) epitopes and two with both Th and cytotoxic T-lymphocyte (CTL) epitopes, on equine infectious anemia virus (EIAV) challenge was evaluated. Peripheral blood mononuclear cells from EIAV lipopeptide-immunized horses had significant proliferative responses to Th peptides compared with those preimmunization, and the responses were attributed to significant responses to peptides Gag from positions 221 to 245 (Gag 221-245), Gag 250-269, and Pol 326-347; however, there were no consistent CTL responses. The significant proliferative responses in the EIAV lipopeptide-immunized horses allowed testing of the hypothesis that Th responses to immunization would enhance Th and CTL responses following EIAV challenge and lessen the viral load and the severity of clinical disease. The EIAV lipopeptide-immunized group did have a significant increase in proliferative responses to Th peptides 1 week after virus challenge, whereas the control group did not. Two weeks after challenge, a significant CTL response to virus-infected cell targets occurred in the EIAV lipopeptide-immunized group compared to that in the control group. These Th and CTL responses did not significantly alter either the number of viral RNA copies/ml or disease severity. Thus, lipopeptide-induced proliferative responses and enhanced Th and CTL responses early after virus challenge were unable to control challenge virus load and clinical disease.

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Title: Lymphocyte proliferation responses induced to broadly reactive Th peptides did not protect against equine infectious anemia virus challenge
Creators: Darrilyn G Fraser - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA
Steve R Leib
Bao Shan Zhang
Robert H Mealey
Wendy C Brown
Travis C McGuire
Publication Details: Clinical and diagnostic laboratory immunology, Vol.12(8), pp.983-993
Academic Unit: Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
Publisher: United States
Grant note: AI47660 / NIAID NIH HHS F32 AI010528 / NIAID NIH HHS AI24291 / NIAID NIH HHS AI10528 / NIAID NIH HHS
Identifiers: 99900546858001842
Language: English
Resource Type: Journal article