Journal article
Lymphocytes and Macrophages Are Infected by Theileria equi, but T Cells and B Cells Are Not Required to Establish Infection In Vivo
PloS one, Vol.8(10), pp.e76996-e76996
10/07/2013
Handle:
https://hdl.handle.net/2376/105822
PMCID: PMC3792048
PMID: 24116194
Abstract
Theileria equi
has a biphasic life cycle in horses, with a period of intraleukocyte development followed by patent erythrocytic parasitemia that causes acute and sometimes fatal hemolytic disease. Unlike
Theileria spp
. that infect cattle (
Theileria parva
and
Theileria annulata
), the intraleukocyte stage (schizont) of
Theileria equi
does not cause uncontrolled host cell proliferation or other significant pathology. Nevertheless, schizont-infected leukocytes are of interest because of their potential to alter host cell function and because immune responses directed against this stage could halt infection and prevent disease. Based on cellular morphology,
Theileria equi
has been reported to infect lymphocytes
in vivo
and
in vitro
, but the specific phenotype of schizont-infected cells has yet to be defined. To resolve this knowledge gap in
Theileria equi
pathogenesis, peripheral blood mononuclear cells were infected
in vitro
and the phenotype of infected cells determined using flow cytometry and immunofluorescence microscopy. These experiments demonstrated that the host cell range of
Theileria equi
was broader than initially reported and included B lymphocytes, T lymphocytes and monocyte/macrophages. To determine if B and T lymphocytes were required to establish infection
in vivo
, horses affected with severe combined immunodeficiency (SCID), which lack functional B and T lymphocytes, were inoculated with
Theileria equi
sporozoites. SCID horses developed patent erythrocytic parasitemia, indicating that B and T lymphocytes are not necessary to complete the
Theileria equi
life cycle
in vivo
. These findings suggest that the factors mediating
Theileria equi
leukocyte invasion and intracytoplasmic differentiation are common to several leukocyte subsets and are less restricted than for
Theileria annulata
and
Theileria parva
. These data will greatly facilitate future investigation into the relationships between
Theileria equi
leukocyte tropism and pathogenesis, breed susceptibility, and strain virulence.
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Details
- Title
- Lymphocytes and Macrophages Are Infected by Theileria equi, but T Cells and B Cells Are Not Required to Establish Infection In Vivo
- Creators
- Joshua D RamsayMassaro W UetiWendell C JohnsonGlen A ScolesDonald P KnowlesRobert H Mealey
- Publication Details
- PloS one, Vol.8(10), pp.e76996-e76996
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Public Library of Science; San Francisco, USA
- Identifiers
- 99900547269501842
- Language
- English
- Resource Type
- Journal article