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CC BY V4.0, Open Access
Journal article
MLST Genotypes and Antibiotic Resistance of Campylobacter spp. Isolated from Poultry in Grenada
BioMed research international, Vol.2013(2013), pp.794643-794643
2013
Handle:
https://hdl.handle.net/2376/107058
PMCID: PMC3595693
PMID: 23555097
Abstract
This study determined whether multilocus sequence types (MLST) of
Campylobacter
from poultry in 2 farms in Grenada, West Indies, differed by farm, antimicrobial resistance and farm antibiotic use. Farm A used fluoroquinolones in the water and Farm B used tetracyclines. The E-test was used to determine resistance of isolates to seven antibiotics. PCR of the
IpxA
gene confirmed species and MLST was used to characterize 38 isolates. All isolates were either
C. jejuni
or
C. coli
. Farm antibiotic use directly correlated with antimicrobial resistance of
Campylobacter
isolates. Almost 80% of the isolates from Farm A were fluoroquinolone resistant and 17.9% of the isolates from Farm B were fluoroquinolone resistant. All
Campylobacter
isolates from Farm A were tetracycline sensitive, whereas 35.7% of isolates from Farm B were tetracycline resistant. Six previously recognized sequence types (STs) and 2 novel STs were identified. Previously recognized STs were those overwhelmingly reported from poultry and humans globally. Isolates with the same ST did not always have the same antibiotic resistance profile. There was little ST overlap between the farms suggesting that within-farm transmission of
Campylobacter
genotypes may dominate. MLST typing was useful for tracking
Campylobacter
spp. among poultry units and can help elucidate
Campylobacter
host-species population structure and its relevance to human health.
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Details
- Title
- MLST Genotypes and Antibiotic Resistance of Campylobacter spp. Isolated from Poultry in Grenada
- Creators
- Diana Stone - School of Veterinary Medicine, St. George's University, P.O. Box 7, Grenada, West IndiesMargaret Davis - College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USAKatherine Baker - College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USATom Besser - College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USARohini Roopnarine - School of Veterinary Medicine, St. George's University, P.O. Box 7, Grenada, West IndiesRavindra Sharma - School of Veterinary Medicine, St. George's University, P.O. Box 7, Grenada, West Indies
- Publication Details
- BioMed research international, Vol.2013(2013), pp.794643-794643
- Academic Unit
- Paul G. Allen School for Global Animal Health; Department of Veterinary Microbiology and Pathology
- Publisher
- Hindawi Publishing Corporation
- Number of pages
- 10
- Grant note
- Wellcome Trust; European Commission St. George's University Small Research Grant Initiative
- Identifiers
- 99900547065701842
- Language
- English
- Resource Type
- Journal article