Journal article
MLVA typing reveals higher genetic homogeneity among S. Enteritidis strains isolated from food, humans and chickens in Brazil in comparison to the North American Strains
International journal of food microbiology, Vol.162(2), pp.174-181
03/15/2013
Handle:
https://hdl.handle.net/2376/100422
PMID: 23416553
Abstract
Salmonella Enteritidis (S. Enteritidis) is a major causative agent of food-borne gastroenteritis associated with the consumption of contaminated poultry products. In this study we used multilocus variable number of tandem repeats (VNTRs) analysis (MLVA) to discriminate a total of 188 S. Enteritidis strains recovered from human (n=67), food (n=61) and chickens (n=60) during a 24year period (1986 through 2010) in Brazil. MLVA profiles of the 188 strains from Brazil were compared to the MLVA profiles of 100 human clinical (n=52) and poultry-associated (n=48) strains isolated in North America between 1986 and 2008. MLVA typing led to classification of the 288 strains from Brazil and North America into two major clusters named A and B with 35% of similarity. Cluster A consisted of a vast majority of strains isolated from North America (n=71) and only three strains isolated from Brazil which included two pre-pandemic strains (SE5 and SE4). In contrast, cluster B consisted of all of the post-pandemic strains isolated from Brazil (n=185) and fewer strains isolated from North America (n=29). In general, MLVA typing showed that the North American strains were more genetically diverse whereas Brazilian strains were more genetically clonal. The clustering of pre-pandemic strains from Brazil with the North American strains suggests the possibility that the pre-pandemic strains were more likely genetically diverse; however after 1993 a new and prevalent subtype of S. Enteritidis was introduced in this country. This is the first study describing MLVA genotyping of the S. Enteritidis strains isolated from Brazil.
► 288 S. Enteritidis isolates from Brazil and North America were genotyped by MLVA. ► Comparison of MLVA profiles led to classification of the strains into two major clusters. ► North American strains were genetically diverse whereas Brazilian strains were clonal. ► After 1993 a new and prevalent subtype of S. Enteritidis was introduced in Brazil.
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Details
- Title
- MLVA typing reveals higher genetic homogeneity among S. Enteritidis strains isolated from food, humans and chickens in Brazil in comparison to the North American Strains
- Creators
- Fábio Campioni - Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Av. do Café, s/n, 14040-903, Ribeirão Preto, SP, BrazilMargaret Davis - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAMarta Inês C Medeiros - Instituto Adolfo Lutz de Ribeirão Preto, Rua Minas, 877, 14085-410, Ribeirão Preto, SP, BrazilJuliana P Falcão - Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Av. do Café, s/n, 14040-903, Ribeirão Preto, SP, BrazilDevendra H Shah - Paul Allen School for Global Animal Health, Washington State University, Pullman, WA 99164, USA
- Publication Details
- International journal of food microbiology, Vol.162(2), pp.174-181
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900546639301842
- Language
- English
- Resource Type
- Journal article