Journal article
MODULATION OF RAD26 AND RPB9 MEDIATED DNA REPAIR BY DIFFERENT PROMOTER ELEMENTS
The Journal of biological chemistry, Vol.281(48), pp.36643-36651
12/01/2006
Handle:
https://hdl.handle.net/2376/110425
PMCID: PMC1913475
PMID: 17023424
Abstract
Rad26, the yeast homologue of human Cockayne syndrome group B protein, and Rpb9, a nonessential subunit of RNA polymerase II, have been shown to mediate two subpathways of transcription coupled DNA repair in yeast. Here we show that Rad26 and Rpb9 mediated repair in the yeast
GAL1
gene are differently modulated by different promoter elements. The initiation site and efficiency of Rad26 mediated repair in the transcribed strand are determined by the upstream activating sequence (UAS), but not by the TATA or local sequences. The role of UAS in determining the Rad26 mediated repair is not through loading of RNA polymerase II or the transcriptional regulatory complex SAGA. However, both the UAS and TATA sequences are essential for confining Rad26 mediated repair to the transcribed strand. Mutation of the TATA sequence, which greatly reduces transcription, or deletion of the TATA or mutation of the UAS, which completely abolishes transcription, causes Rad26 mediated repair to occur in both strands. Rpb9 mediated repair only occurs in the transcribed strand, and is efficient only in the presence of both TATA and UAS sequences. Our results suggest that Rad26 mediated repair can be either transcription-coupled, provided that a substantial level of transcription is present, or transcription-independent, if the transcription is too low or absent. In contrast, Rpb9 mediated repair is strictly transcription-coupled, and is efficient only when the transcription level is high.
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Details
- Title
- MODULATION OF RAD26 AND RPB9 MEDIATED DNA REPAIR BY DIFFERENT PROMOTER ELEMENTS
- Creators
- Shisheng Li - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803Xuefeng Chen - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803Christine Ruggiero - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803Baojin Ding - Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803Michael J Smerdon - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, WA99164-4660
- Publication Details
- The Journal of biological chemistry, Vol.281(48), pp.36643-36651
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900547787301842
- Language
- English
- Resource Type
- Journal article