MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast

Timothy J Hawkins; Patrick J Hussey; Andrei Smertenko; Renate Schmidt; Soon Ki Park; Daniel Schubert; David Twell

doi:10.1038/ncb844

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MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast

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MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast

Timothy J Hawkins, Patrick J Hussey, Andrei Smertenko, Renate Schmidt, Soon Ki Park, Daniel Schubert and David Twell

Nature cell biology, Vol.4(9), pp.711-714

09/2002

DOI: https://doi.org/10.1038/ncb844

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https://hdl.handle.net/2376/108853

PMID: 12198497

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Abstract

MOR1 is a member of the MAP215 family of microtubule-associated proteins and is required to establish interphase arrays of cortical microtubules in plant cells. Here we show that MOR1 binds microtubules in vivo, localizing to both cortical microtubules and to areas of overlapping microtubules in the phragmoplast. Genetic complementation of the cytokinesis-defective gemini pollen 1-1 (gem1-1) mutation with MOR1 shows that MOR1 (which is synonymous with the protein GEM1) is essential in cytokinesis. Phenotypic analysis of gem1-1 and gem1-2, which contains a T-DNA insertion, confirm that MOR1/GEM1 is essential for regular patterns of cytokinesis. Both the gem1-1 and gem1-2 mutations cause the truncation of the MOR1/GEM1 protein. In addition, the carboxy-terminal domain of the protein, which is absent in both mutants, binds microtubules in vitro. Our data show that MOR1/GEM1 has an essential role in the cytokinetic phragmoplast.

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Title: MOR1/GEM1 has an essential role in the plant-specific cytokinetic phragmoplast
Creators: Timothy J Hawkins - The Integrative Cell Biology laboratory, School of Biological and Biomedical Sciences, University of Durham
Patrick J Hussey - The Integrative Cell Biology laboratory, School of Biological and Biomedical Sciences, University of Durham
Andrei Smertenko - The Integrative Cell Biology laboratory, School of Biological and Biomedical Sciences, University of Durham
Renate Schmidt - Max-Planck-Institute of Molecular Plant Physiology
Soon Ki Park - Department of Biology, University of Leicester, University Road
Daniel Schubert - Max-Planck-Institute of Molecular Plant Physiology
David Twell - Department of Biology, University of Leicester, University Road
Publication Details: Nature cell biology, Vol.4(9), pp.711-714
Academic Unit: Biological Chemistry, Institute of
Identifiers: 99900546894001842
Language: English
Resource Type: Journal article