Journal article
Male tarsi specific odorant-binding proteins in the diving beetle Cybister japonicus sharp
Scientific reports, Vol.6(1), pp.31848-31848
08/22/2016
Handle:
https://hdl.handle.net/2376/100300
PMCID: PMC4992826
PMID: 27545810
Abstract
Odorant binding proteins (OBPs) play critical roles in chemical communication of insects, as they recognize and transport environmental chemical signals to receptors. The diving beetle Cybister japonicus Sharp shows a remarkable sexual dimorphism. The foreleg tarsi of males are equipped with large suction cups, believed to help holding the female during underwater courtship and mating. Here, we identified two OBPs highly and specifically expressed in male tarsi, suggesting important functions of these structures in chemical communication. The first protein, CjapOBP1, exhibits the 6 conserved cysteines motif of classic OBPs, while the second, CjapOBP2, contains only four cysteines and can be assigned to the sub-class of C-minus OBPs. Both proteins were expressed in a bacterial system and the purified recombinant proteins were used to for antibodies preparation. Western Blot analysis showed that CjapOBP1 is predominantly expressed in male tarsi and could be also detected in antennae and palpi of both sexes, while CjapOBP2, besides male tarsi, is also present in testis. Ligand-binding experiments showed a good binding affinity between CjapOBP1, CjapOBP2 and citral and coniferyl aldehyde, respectively. These results support a possible function of these two OBPs in the male foreleg tarsi of diving beetles in chemical communication.
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Details
- Title
- Male tarsi specific odorant-binding proteins in the diving beetle Cybister japonicus sharp
- Creators
- Li-Mei Song - College of Animal Science and Technology, China Agricultural University, Beijing, 100193, ChinaXiang Jiang - HuangPu Entry-Exit Inspection and Quarantine Bureau, Guangdong, 510730, ChinaXue-Min Wang - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 10 0193, ChinaJin-Dong Li - College of Animal Science and Technology, China Agricultural University, Beijing, 100193, ChinaFang Zhu - Department of Entomology, Washington State University, Pullman, WA, 99164, USAXiong-Bing Tu - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaZe-Hua Zhang - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, ChinaLi-Ping Ban - College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
- Publication Details
- Scientific reports, Vol.6(1), pp.31848-31848
- Publisher
- England
- Identifiers
- 99900546511101842
- Language
- English
- Resource Type
- Journal article